Liu L, Xu W, Harrington K A, Emson P C
MRC Molecular Neuroscience Group, Department of Neurobiology, Babraham Institute, Cambridge, UK.
Brain Res Mol Brain Res. 1994 Aug;25(1-2):90-6. doi: 10.1016/0169-328x(94)90282-8.
A synaptic vesicle amine transporter cDNA, termed hSVAT, has been isolated by the reverse transcription and polymerase chain reaction (PCR) technique from human substantia nigra and subsequent screening of a human substantia nigra library. The hSVAT sequence obtained is highly homologous to the rat SVAT sequence (92% homology) and is essentially identical to the human sequence identified recently by Surratt and colleagues [33]. This labelled hSVAT cDNA detected a single band (approximately 5.0 kb) when used as a probe for Northern analysis of human nigral RNA extract. In situ hybridization studies using hSVAT specific antisense oligonucleotides showed a strong hybridization signal concentrated over the cells of the substantia nigra pars compacta. This cDNA sequence when expressed in chinese hamster ovary (CHO) cells conferred resistance to MPP+ the toxic metabolite of MPTP and cells containing it accumulated dopamine.
一种名为hSVAT的突触囊泡胺转运体cDNA，已通过逆转录和聚合酶链反应（PCR）技术从人黑质中分离出来，并随后筛选了人黑质文库。获得的hSVAT序列与大鼠SVAT序列高度同源（92%同源性），并且与Surratt及其同事最近鉴定的人类序列基本相同[33]。当用作人黑质RNA提取物Northern分析的探针时，这种标记的hSVAT cDNA检测到一条单带（约5.0 kb）。使用hSVAT特异性反义寡核苷酸的原位杂交研究显示，强烈的杂交信号集中在黑质致密部的细胞上。该cDNA序列在中国仓鼠卵巢（CHO）细胞中表达时，赋予了对MPTP的有毒代谢物MPP+的抗性，并且含有它的细胞积累了多巴胺。
