致癌物N-2-乙酰氨基芴对移码诱变中间体稳定作用的核磁共振证据。
NMR evidence of the stabilisation by the carcinogen N-2-acetylaminofluorene of a frameshift mutagenesis intermediate.
作者信息
Milhé C, Dhalluin C, Fuchs R P, Lefèvre J F
机构信息
CNRS-UPR 9003 Cancérogenèse et Mutagenèse Moléculaire et Structurale, ESBS, Pôle API, Illkirch-Graffenstaden, France.
出版信息
Nucleic Acids Res. 1994 Nov 11;22(22):4646-52. doi: 10.1093/nar/22.22.4646.
Abstract
Two heteroduplexes d(C1A2C3T4C5G6C7A8C9A10C11)-d (G12T13G14T15G16G17A18G19T20G21) containing a bulged guanine either unmodified or modified with the carcinogen N-2-acetylaminofluorene (AAF) have been studied by nuclear magnetic resonance (NMR) as models of slipped mutagenic intermediates (SMI). Conformational equilibria are observed in both the unmodified and the AAF-modified heteroduplexes. The major conformation of the unmodified duplex is one where the extra guanine is stacked in the helix and the major conformation of the AAF-modified heteroduplex is one where the AAF is external to the helix. Unusual sugar proton chemical shifts of C5- and G6-AAF indicate that the AAF ring is pointing out in the 5' direction. A strong increase in the modified heteroduplex melting temperature (+15 degrees C) is observed. Moreover, in contrast to the unmodified heteroduplex, which shows extensive melting in the vicinity of the bulged guanine, the base pairs around the bulge in the AAF-modified heteroduplex remain paired at temperatures up to 30 degrees C. This exceptional stability of the site around the bulged modified guanine is suggested to be responsible for the high rate of -1 mutation induced by AAF at repetitive sequences.
摘要
两个异源双链体d（C1A2C3T4C5G6C7A8C9A10C11）-d（G12T13G14T15G16G17A18G19T20G21），其中含有一个未修饰或用致癌物N-2-乙酰氨基芴（AAF）修饰的凸起鸟嘌呤，已通过核磁共振（NMR）作为滑动诱变中间体（SMI）的模型进行了研究。在未修饰和AAF修饰的异源双链体中均观察到构象平衡。未修饰双链体的主要构象是额外的鸟嘌呤堆积在螺旋中的构象，而AAF修饰的异源双链体的主要构象是AAF位于螺旋外部的构象。C5-和G6-AAF不寻常的糖质子化学位移表明AAF环指向5'方向。观察到修饰的异源双链体解链温度有显著升高（+15摄氏度）。此外，与未修饰的异源双链体在凸起鸟嘌呤附近表现出广泛解链不同，AAF修饰的异源双链体中凸起周围的碱基对在高达30摄氏度的温度下仍保持配对。凸起修饰鸟嘌呤周围位点的这种异常稳定性被认为是导致AAF在重复序列处诱导-1突变的高发生率的原因。
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