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Nucleic Acids Res. 1994 Nov 11;22(22):4646-52. doi: 10.1093/nar/22.22.4646.
2
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3
DNA adduct-induced stabilization of slipped frameshift intermediates within repetitive sequences: implications for mutagenesis.DNA加合物诱导的重复序列内滑动框移中间体的稳定：对诱变的影响。
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Binding and incision activities of UvrABC excinuclease on slipped DNA intermediates that generate frameshift mutations.UvrABC核酸外切酶对产生移码突变的滑动DNA中间体的结合和切割活性。
J Mol Biol. 1996 Apr 19;257(5):970-6. doi: 10.1006/jmbi.1996.0216.
5
Structural characterization of an N-acetyl-2-aminofluorene (AAF) modified DNA oligomer by NMR, energy minimization, and molecular dynamics.通过核磁共振、能量最小化和分子动力学对N-乙酰-2-氨基芴（AAF）修饰的DNA寡聚物进行结构表征。
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Biochemistry. 1999 Aug 17;38(33):10843-54. doi: 10.1021/bi9912138.
10
Solution structure of the aminofluorene [AF]-external conformer of the anti-[AF]-C8-dG adduct opposite dC in a DNA duplex.DNA双链中与dC相对的反式-[氨基芴（AF）]-C8-dG加合物的AF-外部构象异构体的溶液结构。
Biochemistry. 1998 Jan 6;37(1):95-106. doi: 10.1021/bi972258g.

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Structure and thermodynamic insights on acetylaminofluorene-modified deletion DNA duplexes as models for frameshift mutagenesis.乙酰氨基芴修饰的缺失 DNA 双链结构与热力学研究——作为移码突变模型。
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Base-displaced intercalated structure of the food mutagen 2-amino-3-methylimidazo[4,5-f]quinoline in the recognition sequence of the NarI restriction enzyme, a hotspot for -2 bp deletions.食品诱变剂2-氨基-3-甲基咪唑[4,5-f]喹啉在NarI限制酶识别序列中的碱基置换插入结构，即-2bp缺失的热点区域。
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Mechanism of DNA polymerase II-mediated frameshift mutagenesis.DNA聚合酶II介导的移码突变机制。
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本文引用的文献

1
Structural characterization of an N-acetyl-2-aminofluorene (AAF) modified DNA oligomer by NMR, energy minimization, and molecular dynamics.通过核磁共振、能量最小化和分子动力学对N-乙酰-2-氨基芴（AAF）修饰的DNA寡聚物进行结构表征。
Biochemistry. 1993 Mar 16;32(10):2481-97. doi: 10.1021/bi00061a005.
2
DNA adduct-induced stabilization of slipped frameshift intermediates within repetitive sequences: implications for mutagenesis.DNA加合物诱导的重复序列内滑动框移中间体的稳定：对诱变的影响。
Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):5989-93. doi: 10.1073/pnas.90.13.5989.
3
NMR structural studies of a 15-mer DNA duplex from a ras protooncogene modified with the carcinogen 2-aminofluorene: conformational heterogeneity.对来自ras原癌基因的一段经致癌物2-氨基芴修饰的15聚体DNA双链体的核磁共振结构研究：构象异质性
Biochemistry. 1994 Feb 15;33(6):1373-84. doi: 10.1021/bi00172a013.
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Use of single-turnover kinetics to study bulky adduct bypass by T7 DNA polymerase.利用单轮动力学研究T7 DNA聚合酶对大体积加合物的旁路作用。
Biochemistry. 1994 Jan 25;33(3):764-72. doi: 10.1021/bi00169a018.
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2-Aminofluorene modified DNA duplex exists in two interchangeable conformations.
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6
Carcinogen-induced mutation spectrum in wild-type, uvrA and umuC strains of Escherichia coli. Strain specificity and mutation-prone sequences.致癌物诱导的大肠杆菌野生型、uvrA和umuC菌株中的突变谱。菌株特异性和易突变序列。
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Application of phase sensitive two-dimensional correlated spectroscopy (COSY) for measurements of 1H-1H spin-spin coupling constants in proteins.相敏二维相关光谱法（COSY）在蛋白质中¹H-¹H自旋-自旋耦合常数测量中的应用。
Biochem Biophys Res Commun. 1983 Jun 29;113(3):967-74. doi: 10.1016/0006-291x(83)91093-8.
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Frameshift mutations and the genetic code. This paper is dedicated to Professor Theodosius Dobzhansky on the occasion of his 66th birthday.移码突变与遗传密码。本文谨献给西奥多修斯·杜布赞斯基教授，以庆祝他66岁生日。
Cold Spring Harb Symp Quant Biol. 1966;31:77-84. doi: 10.1101/sqb.1966.031.01.014.
9
Structural model for an oligonucleotide containing a bulged guanosine by NMR and energy minimization.通过核磁共振和能量最小化得到的含凸起鸟苷的寡核苷酸的结构模型。
Biochemistry. 1988 May 3;27(9):3130-41. doi: 10.1021/bi00409a004.
10
1H NMR study of self-association and restricted internal rotation of the C8-substituted deoxyguanosine 5'-monophosphate adduct of the carcinogen 2-(acetylamino)fluorene.致癌物2-（乙酰氨基）芴的C8-取代脱氧鸟苷5'-单磷酸加合物的自缔合和受限内旋转的1H NMR研究
J Biomol Struct Dyn. 1986 Apr;3(5):935-48. doi: 10.1080/07391102.1986.10508475.

致癌物N-2-乙酰氨基芴对移码诱变中间体稳定作用的核磁共振证据。

NMR evidence of the stabilisation by the carcinogen N-2-acetylaminofluorene of a frameshift mutagenesis intermediate.

作者信息

Milhé C, Dhalluin C, Fuchs R P, Lefèvre J F

机构信息

CNRS-UPR 9003 Cancérogenèse et Mutagenèse Moléculaire et Structurale, ESBS, Pôle API, Illkirch-Graffenstaden, France.

出版信息

Nucleic Acids Res. 1994 Nov 11;22(22):4646-52. doi: 10.1093/nar/22.22.4646.

DOI:10.1093/nar/22.22.4646
PMID:7984413
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC308513/
Abstract

Two heteroduplexes d(C1A2C3T4C5G6C7A8C9A10C11)-d (G12T13G14T15G16G17A18G19T20G21) containing a bulged guanine either unmodified or modified with the carcinogen N-2-acetylaminofluorene (AAF) have been studied by nuclear magnetic resonance (NMR) as models of slipped mutagenic intermediates (SMI). Conformational equilibria are observed in both the unmodified and the AAF-modified heteroduplexes. The major conformation of the unmodified duplex is one where the extra guanine is stacked in the helix and the major conformation of the AAF-modified heteroduplex is one where the AAF is external to the helix. Unusual sugar proton chemical shifts of C5- and G6-AAF indicate that the AAF ring is pointing out in the 5' direction. A strong increase in the modified heteroduplex melting temperature (+15 degrees C) is observed. Moreover, in contrast to the unmodified heteroduplex, which shows extensive melting in the vicinity of the bulged guanine, the base pairs around the bulge in the AAF-modified heteroduplex remain paired at temperatures up to 30 degrees C. This exceptional stability of the site around the bulged modified guanine is suggested to be responsible for the high rate of -1 mutation induced by AAF at repetitive sequences.

摘要

两个异源双链体d（C1A2C3T4C5G6C7A8C9A10C11）-d（G12T13G14T15G16G17A18G19T20G21），其中含有一个未修饰或用致癌物N-2-乙酰氨基芴（AAF）修饰的凸起鸟嘌呤，已通过核磁共振（NMR）作为滑动诱变中间体（SMI）的模型进行了研究。在未修饰和AAF修饰的异源双链体中均观察到构象平衡。未修饰双链体的主要构象是额外的鸟嘌呤堆积在螺旋中的构象，而AAF修饰的异源双链体的主要构象是AAF位于螺旋外部的构象。C5-和G6-AAF不寻常的糖质子化学位移表明AAF环指向5'方向。观察到修饰的异源双链体解链温度有显著升高（+15摄氏度）。此外，与未修饰的异源双链体在凸起鸟嘌呤附近表现出广泛解链不同，AAF修饰的异源双链体中凸起周围的碱基对在高达30摄氏度的温度下仍保持配对。凸起修饰鸟嘌呤周围位点的这种异常稳定性被认为是导致AAF在重复序列处诱导-1突变的高发生率的原因。