Ligation-independent cloning of PCR products with primers containing nonbase residues.
作者信息
Kaluz S, Flint A P
机构信息
University of Nottingham, Sutton Bonington Campus, Loughborough, UK.
出版信息
Nucleic Acids Res. 1994 Nov 11;22(22):4845. doi: 10.1093/nar/22.22.4845.
Abstract
摘要
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本文引用的文献
1
Exonuclease III induced ligase-free directional subcloning of PCR products.核酸外切酶III诱导的PCR产物无连接酶定向亚克隆
Nucleic Acids Res. 1993 Nov 25;21(23):5528-9. doi: 10.1093/nar/21.23.5528.
2
Incorporation of nonbase residues into synthetic oligonucleotides and their use in the PCR.
Genet Anal Tech Appl. 1993 Apr;10(2):61-5. doi: 10.1016/1050-3862(93)90046-l.
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Nucleotide insertion kinetics opposite abasic lesions in DNA.DNA中无碱基损伤对面的核苷酸插入动力学。
J Biol Chem. 1987 May 15;262(14):6864-70.
4
Ligation-independent cloning of PCR products (LIC-PCR).聚合酶链式反应（PCR）产物的不依赖连接酶克隆（LIC-PCR）。
Nucleic Acids Res. 1990 Oct 25;18(20):6069-74. doi: 10.1093/nar/18.20.6069.
5
Functional cloning vectors for use in directional cDNA cloning using cohesive ends produced with T4 DNA polymerase.用于使用T4 DNA聚合酶产生的粘性末端进行定向cDNA克隆的功能性克隆载体。
Gene. 1992 Mar 15;112(2):147-55. doi: 10.1016/0378-1119(92)90370-5.
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