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Exonuclease III induced ligase-free directional subcloning of PCR products.核酸外切酶III诱导的PCR产物无连接酶定向亚克隆
Nucleic Acids Res. 1993 Nov 25;21(23):5528-9. doi: 10.1093/nar/21.23.5528.
2
An improved ligase-free method for directional subcloning of PCR amplified DNA.一种改进的用于PCR扩增DNA定向亚克隆的无连接酶方法。
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Directional cloning of PCR products using exonuclease III.使用核酸外切酶III对PCR产物进行定向克隆。
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4
Rapid (ligase-free) subcloning of PCR products.
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Restrictionless cloning.无限制克隆
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Ligase-free subcloning: a versatile method to subclone polymerase chain reaction (PCR) products in a single day.
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PCR-induced (ligase-free) subcloning: a rapid reliable method to subclone polymerase chain reaction (PCR) products.PCR诱导（无连接酶）亚克隆：一种快速可靠的亚克隆聚合酶链反应（PCR）产物的方法。
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Simple and efficient cloning of small polymerase chain reaction-generated DNA products.
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[A method for PCR product cloning based on exonuclease III].
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TA-GC cloning: A new simple and versatile technique for the directional cloning of PCR products for recombinant protein expression.TA-GC克隆：一种用于重组蛋白表达的PCR产物定向克隆的新型简单通用技术。
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Patch cloning method for multiple site-directed and saturation mutagenesis.多位点定点突变和饱和突变的补丁克隆方法。
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Ribosomal binding site switching: an effective strategy for high-throughput cloning constructions.核糖体结合位点切换：高通量克隆构建的有效策略。
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A one-step cloning method for the construction of somatic cell gene targeting vectors: application to production of human knockout cell lines.一步克隆法构建体细胞基因打靶载体：在生产人基因敲除细胞系中的应用。
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The terminal 5' phosphate and proximate phosphorothioate promote ligation-independent cloning.末端 5' 磷酸和临近的硫代磷酸酯促进非连接依赖性克隆。
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Ligation independent cloning irrespective of restriction site compatibility.不依赖连接的克隆，与限制酶切位点兼容性无关。
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Coexpression of nuclear receptor partners increases their solubility and biological activities.核受体伴侣的共表达增加了它们的溶解度和生物学活性。
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本文引用的文献

1
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.改良的M13噬菌体克隆载体和宿主菌株：M13mp18和pUC19载体的核苷酸序列。
Gene. 1985;33(1):103-19. doi: 10.1016/0378-1119(85)90120-9.
2
Site-directed mutagenesis by overlap extension using the polymerase chain reaction.利用聚合酶链反应通过重叠延伸进行定点诱变。
Gene. 1989 Apr 15;77(1):51-9. doi: 10.1016/0378-1119(89)90358-2.
3
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.使用热稳定DNA聚合酶进行引物引导的DNA酶促扩增。
Science. 1988 Jan 29;239(4839):487-91. doi: 10.1126/science.2448875.
4
Directional cloning of PCR products using exonuclease III.使用核酸外切酶III对PCR产物进行定向克隆。
Nucleic Acids Res. 1992 Aug 25;20(16):4369-70. doi: 10.1093/nar/20.16.4369.

Exonuclease III induced ligase-free directional subcloning of PCR products.

作者信息

Hsiao K

机构信息

Department of Molecular Immunology, Bristol-Myers Squibb Pharmaceutical Research Institute-Seattle, WA 98121.

出版信息

Nucleic Acids Res. 1993 Nov 25;21(23):5528-9. doi: 10.1093/nar/21.23.5528.

DOI:10.1093/nar/21.23.5528
PMID:8265374
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC310601/
Abstract
摘要