人血浆中循环心房利钠肽的分子形式及其代谢产物。

Molecular forms of circulating atrial natriuretic peptides in human plasma and their metabolites.

作者信息

Gower W R, Chiou S, Skolnick K A, Vesely D L

机构信息

Department of Biochemistry, University of South Florida Health Sciences Center, and James A. Haley Veterans Hospital, Tampa 33612.

出版信息

Peptides. 1994;15(5):861-7. doi: 10.1016/0196-9781(94)90043-4.

DOI:10.1016/0196-9781(94)90043-4

PMID:7984506

Abstract

High performance gel permeation chromatography (HP-GPC) followed by four radioimmunoassays (RIAs) devised to amino acids (a.a.) 1-30, 31-67, 79-98, and 99-126 of the 126 a.a. atrial natriuretic factor (ANF) prohormone revealed that the proANF(1-30) assay immunoreactivity in plasma is 50% proANF(1-30) and 50% proANF(1-98). The HP-GPC evaluation of plasma followed by proANF(31-67) and ANF [i.e., proANF(99-126)] assays revealed that proANF(31-67) and ANF circulate as distinct peptides. the HP-GPC plasma examination followed by proANF(79-98) assay immunologically recognized three peaks in plasma consistent with proANF(1-98), -(68-98), and -(79-98). Similar HP-GPC evaluation of urine followed by these RIAs indicated that the proANF(1-30), -(79-98), and ANF assays only recognize 500 mol.wt. or less peptides, and the proANF(31-67) RIA recognizes a nearly intact proANF(31-67) with only two to three amino acids removed during processing of this peptide.

摘要

采用高效凝胶渗透色谱法（HP-GPC），随后对126个氨基酸的心房利钠因子（ANF）前体激素的1-30、31-67、79-98和99-126氨基酸进行四种放射免疫分析（RIA），结果显示血浆中proANF(1-30)分析的免疫反应性为50%的proANF(1-30)和50%的proANF(1-98)。对血浆进行HP-GPC评估，随后进行proANF(31-67)和ANF[即proANF(99-126)]分析，结果显示proANF(31-67)和ANF以不同的肽形式循环。对血浆进行HP-GPC检测，随后进行proANF(79-98)分析，免疫检测到血浆中有三个峰，与proANF(1-98)、-(68-98)和-(79-98)一致。对尿液进行类似的HP-GPC评估，随后进行这些RIA分析，结果表明proANF(1-30)、-(79-98)和ANF分析仅识别分子量为500道尔顿或更小的肽，而proANF(31-67)RIA识别的是几乎完整的proANF(31-67)，在该肽的加工过程中仅去除了两到三个氨基酸。