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Effect of oxygen abstraction on the peroxidase-luminol-perborate system: relevance to the HRP enhanced chemiluminescence mechanism.

作者信息

Cercek B, Cercek B, Roby K, Cercek L

机构信息

Beckman Instruments Inc., Diagnostic Systems Group, Brea, California.

出版信息

J Biolumin Chemilumin. 1994 Jul-Aug;9(4):273-7. doi: 10.1002/bio.1170090405.

DOI:10.1002/bio.1170090405
PMID:7985528
Abstract

Abstraction of oxygen from the HRP enhanced chemiluminescence system has no significant effect on the chemiluminescence generated. It is, therefore, proposed that in the peroxidase-luminol-perborate system at pH 7.3, chemiluminescence is generated by a direct reaction of diazaquinones with hydrogen peroxide and not, as generally assumed, from the reaction of luminol radicals with the molecular oxygen.

摘要

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