Band M, Ron M
Institute of Animal Sciences, ARO, Volcani Center, Bet Dagan, Israel.
Anim Genet. 1994 Aug;25(4):281-3. doi: 10.1111/j.1365-2052.1994.tb00206.x.
A bovine genomic library was screened for the presence of (AGC)n repeats. All isolated AGC repeats were located adjacent to the 3' end of bovine short interspersed nuclear elements (SINE). Polymerase chain reactions (PCR) using either two unique primers or one unique and one SINE primer produced high-resolution products without the secondary artifact ladders typical of dinucleotide microsatellites. Four AGC microsatellites were found to be polymorphic with 2-4 alleles each and polymorphism information context (PIC) values ranging between 0.26 and 0.49. One microsatellite, ARO25, was mapped to chromosome 26 with the CSIRO reference families. Because of their strong association with AGC repeats and high frequency in the genome, SINE-3' PCR may prove to be a novel source of polymorphic trinucleotide markers in the bovine genome.
对一个牛基因组文库进行筛选，以寻找(AGC)n重复序列。所有分离出的AGC重复序列都位于牛短散在核元件(SINE)的3'端附近。使用两个独特引物或一个独特引物与一个SINE引物进行聚合酶链反应(PCR)，产生了高分辨率产物，没有二核苷酸微卫星典型的二级伪带。发现四个AGC微卫星具有多态性，每个微卫星有2 - 4个等位基因，多态性信息含量(PIC)值在0.26至0.49之间。利用CSIRO参考家系将一个微卫星ARO25定位到26号染色体上。由于它们与AGC重复序列的紧密关联以及在基因组中的高频率，SINE - 3' PCR可能被证明是牛基因组中多态性三核苷酸标记的一个新来源。
