猪多态性微卫星位点的特征分析

Characterization of porcine polymorphic microsatellite loci.

作者信息

Coppieters W, Van de Weghe A, Peelman L, Depicker A, Van Zeveren A, Bouquet Y

机构信息

Department of Animal Nutrition, Production and Ethology, Faculty of Veterinary Medicine, University of Ghent, Merelbeke, Belgium.

出版信息

Anim Genet. 1993 Jun;24(3):163-70. doi: 10.1111/j.1365-2052.1993.tb00281.x.

DOI:10.1111/j.1365-2052.1993.tb00281.x

PMID:8103299

Abstract

Twenty-seven (CA)n and two (GA)n microsatellite clones were isolated out of a size-selected genomic pig library. These were sequenced and the number of uninterrupted dinucleotides was found to range from 12 to 26. Flanking primers were chosen for 11 dinucleotide repeats and optimal conditions for polymerase chain reaction (PCR) amplifications were established. Different microsatellite loci were amplified simultaneously by combining primer sets. Related and unrelated pigs were screened for length polymorphisms of the different microsatellite loci. The polymorphic information content (PIC) of these loci ranged between 0.62 and 0.83. Segregation studies in pig reference families established Mendelian inheritance. Locus S0022 was found to be X-linked.

摘要

从一个经过大小选择的猪基因组文库中分离出了27个(CA)n和2个(GA)n微卫星克隆。对这些克隆进行测序后发现，不间断二核苷酸的数量在12至26之间。为11个二核苷酸重复序列选择了侧翼引物，并确定了聚合酶链反应(PCR)扩增的最佳条件。通过组合引物对同时扩增不同的微卫星位点。对相关和不相关的猪进行了不同微卫星位点长度多态性的筛选。这些位点的多态信息含量(PIC)在0.62至0.83之间。在猪参考家系中的分离研究确定了孟德尔遗传。发现位点S0022是X连锁的。