预先吸附的乳蛋白对单核细胞增生李斯特菌黏附疏水性和亲水性二氧化硅表面的影响。
Influence of preadsorbed milk proteins on adhesion of Listeria monocytogenes to hydrophobic and hydrophilic silica surfaces.
作者信息
al-Makhlafi H, McGuire J, Daeschel M
机构信息
Department of Food Science & Technology, Oregon State University, Corvallis 97331-3906.
出版信息
Appl Environ Microbiol. 1994 Oct;60(10):3560-5. doi: 10.1128/aem.60.10.3560-3565.1994.
The adsorption of beta-lactoglobulin, bovine serum albumin, alpha-lactalbumin, and beta-casein for 8 h and beta-lactoglobulin and bovine serum albumin for 1 h at silanized silica surfaces of low and high hydrophobicity, followed by incubation in buffer and contact with Listeria monocytogenes, resulted in different numbers of cells adhered per unit of surface area. Adhesion to both surfaces was greatest when beta-lactoglobulin was present and was lowest when bovine serum albumin was present. Preadsorption of alpha-lactalbumin and beta-casein showed an intermediate effect on cell adhesion. Adsorption of beta-lactoglobulin for 1 h resulted in a generally lower number of cells adhered compared with the 8-h adsorption time, while the opposite result was observed with respect to bovine serum albumin. The adhesion data were explainable in terms of the relative rates of arrival to the surface and postadsorptive conformational change among the proteins, in addition to the extent of surface coverage in each case.
在低疏水性和高疏水性的硅烷化二氧化硅表面，β-乳球蛋白、牛血清白蛋白、α-乳白蛋白和β-酪蛋白吸附8小时，β-乳球蛋白和牛血清白蛋白吸附1小时，随后在缓冲液中孵育并与单核细胞增生李斯特菌接触，结果导致每单位表面积附着的细胞数量不同。当存在β-乳球蛋白时，在两个表面上的附着力最大，而当存在牛血清白蛋白时，附着力最低。α-乳白蛋白和β-酪蛋白的预吸附对细胞粘附显示出中间效应。与吸附8小时相比，β-乳球蛋白吸附1小时导致附着的细胞数量通常较少，而对于牛血清白蛋白则观察到相反的结果。除了每种情况下的表面覆盖程度外，粘附数据还可以根据蛋白质到达表面的相对速率和吸附后构象变化来解释。
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