Cole A A, Chubinskaya S, Luchene L J, Chlebek K, Orth M W, Greenwald R A, Kuettner K E, Schmid T M
Rush Medical College, Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612.
Arthritis Rheum. 1994 Dec;37(12):1727-34. doi: 10.1002/art.1780371204.
The effects of doxycycline were tested in an in vitro system in which the cartilages of embryonic avian tibias are completely degraded.
Tibias were cultured with 5, 20, or 40 microgram/ml doxycycline. Control tibias were cultured without doxycycline. Conditioned media and tissue sections were examined for enzyme activity and matrix loss.
Cartilages were not resorbed in the presence of doxycycline, whereas control cartilages were completely degraded. Collagen degradation was reduced in association with treatment with doxycycline at all doses studied. Higher concentrations of doxycycline reduced collagenase and gelatinase activity and prevented proteoglycan loss, cell death, and deposition of type X collagen in the cartilage matrix; in addition, treatment with doxycycline at higher concentrations caused increases in the length of the hypertrophic region.
The effects of doxycycline extend beyond inhibition of the proteolytic enzymes by stimulating cartilage growth and disrupting the terminal differentiation of chondrocytes.
在一个胚胎期禽类胫骨软骨完全降解的体外系统中测试强力霉素的作用。
将胫骨用5、20或40微克/毫升的强力霉素培养。对照胫骨在无强力霉素的情况下培养。检测条件培养基和组织切片的酶活性及基质损失情况。
在强力霉素存在的情况下软骨未被吸收，而对照软骨则完全降解。在所研究的所有剂量下，与强力霉素治疗相关的胶原蛋白降解均减少。更高浓度的强力霉素降低了胶原酶和明胶酶的活性，并防止了蛋白聚糖的损失、细胞死亡以及X型胶原蛋白在软骨基质中的沉积；此外，更高浓度的强力霉素治疗导致肥大区域长度增加。
强力霉素的作用不仅限于抑制蛋白水解酶，还通过刺激软骨生长和破坏软骨细胞的终末分化来发挥作用。
