Bologna C, Edno L, Anaya J M, Canovas F, Vanden Berghe M, Jorgensen C, Galtier M, Combe B, Bressolle F, Sany J
CHU Gui-de-Chauliac, Montpellier, France.
Arthritis Rheum. 1994 Dec;37(12):1770-3. doi: 10.1002/art.1780371210.
To determine methotrexate (MTX) concentrations in the synovial membrane (SM) and cortical and trabecular bone of rheumatoid arthritis (RA) patients.
Ten RA patients (9 women, 1 man; mean +/- SD age 49.2 +/- 10.6, mean disease duration 13.2 +/- 9.9 years) undergoing surgical procedures for rheumatoid articular lesions participated in this study. Mean +/- SD MTX treatment duration was 26.4 +/- 21.3 months. The day preceding surgery, 10 mg of MTX was administered intramuscularly. During surgery, a mean +/- SD of 19.7 +/- 2.6 hours after MTX administration, SM, bone fragments, and blood were collected simultaneously. MTX was assayed by fluorescence polarization immunoassay in plasma and tissues.
The mean +/- SD plasma concentration was 0.0252 +/- 0.01 nmoles/ml at the time of tissue sampling. The mean MTX concentration in SM was 0.285 +/- 0.159 nmoles/gm. The mean MTX concentrations in trabecular and cortical bone were 0.292 +/- 0.164 and 0.286 +/- 0.126 nmoles/gm, respectively.
After intramuscular administration, high MTX concentrations are found in SM and cortical and trabecular bone of RA patients.
测定类风湿关节炎（RA）患者滑膜（SM）、皮质骨和小梁骨中的甲氨蝶呤（MTX）浓度。
10例因类风湿关节病变接受外科手术的RA患者（9例女性，1例男性；平均±标准差年龄49.2±10.6岁，平均病程13.2±9.9年）参与本研究。MTX平均治疗时间±标准差为26.4±21.3个月。手术前一天，肌肉注射10 mg MTX。手术期间，在MTX给药后平均±标准差19.7±2.6小时，同时采集SM、骨碎片和血液。采用荧光偏振免疫分析法测定血浆和组织中的MTX。
组织采样时血浆平均±标准差浓度为0.0252±0.01纳摩尔/毫升。SM中MTX平均浓度为0.285±0.159纳摩尔/克。小梁骨和皮质骨中MTX平均浓度分别为0.292±0.164和0.286±0.126纳摩尔/克。
肌肉注射后，RA患者的SM、皮质骨和小梁骨中发现高MTX浓度。
