类风湿关节炎患者滑膜、小梁骨和皮质骨中的甲氨蝶呤浓度。

Methotrexate concentrations in synovial membrane and trabecular and cortical bone in rheumatoid arthritis patients.

作者信息

Bologna C, Edno L, Anaya J M, Canovas F, Vanden Berghe M, Jorgensen C, Galtier M, Combe B, Bressolle F, Sany J

机构信息

CHU Gui-de-Chauliac, Montpellier, France.

出版信息

Arthritis Rheum. 1994 Dec;37(12):1770-3. doi: 10.1002/art.1780371210.

DOI:10.1002/art.1780371210

PMID:7986223

Abstract

OBJECTIVE

To determine methotrexate (MTX) concentrations in the synovial membrane (SM) and cortical and trabecular bone of rheumatoid arthritis (RA) patients.

METHODS

Ten RA patients (9 women, 1 man; mean +/- SD age 49.2 +/- 10.6, mean disease duration 13.2 +/- 9.9 years) undergoing surgical procedures for rheumatoid articular lesions participated in this study. Mean +/- SD MTX treatment duration was 26.4 +/- 21.3 months. The day preceding surgery, 10 mg of MTX was administered intramuscularly. During surgery, a mean +/- SD of 19.7 +/- 2.6 hours after MTX administration, SM, bone fragments, and blood were collected simultaneously. MTX was assayed by fluorescence polarization immunoassay in plasma and tissues.

RESULTS

The mean +/- SD plasma concentration was 0.0252 +/- 0.01 nmoles/ml at the time of tissue sampling. The mean MTX concentration in SM was 0.285 +/- 0.159 nmoles/gm. The mean MTX concentrations in trabecular and cortical bone were 0.292 +/- 0.164 and 0.286 +/- 0.126 nmoles/gm, respectively.

CONCLUSION

After intramuscular administration, high MTX concentrations are found in SM and cortical and trabecular bone of RA patients.

摘要

目的

测定类风湿关节炎（RA）患者滑膜（SM）、皮质骨和小梁骨中的甲氨蝶呤（MTX）浓度。

方法

10例因类风湿关节病变接受外科手术的RA患者（9例女性，1例男性；平均±标准差年龄49.2±10.6岁，平均病程13.2±9.9年）参与本研究。MTX平均治疗时间±标准差为26.4±21.3个月。手术前一天，肌肉注射10 mg MTX。手术期间，在MTX给药后平均±标准差19.7±2.6小时，同时采集SM、骨碎片和血液。采用荧光偏振免疫分析法测定血浆和组织中的MTX。