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酶联免疫吸附测定(ELISA)。

The enzyme-linked immunosorbent assay (ELISA).

出版信息

Bull World Health Organ. 1976;54(2):129-39.

Abstract

The enzyme-linked immunosorbent assay "ELISA" developed in recent years represents a significant addition to existing serological tools. Encouraging preliminary results obtained through its application to a number of parasitic diseases during the last two years indicate the value of further investigations and trials which will permit a true evaluation.Although the technique is easy to perform and quite sensitive, there are certain problems to be solved before it becomes widely usable. In the present Memorandum the technical details are given and the advantages and shortcomings of the procedure are discussed. Present applications and future prospects are reviewed.

摘要

近年来开发的酶联免疫吸附测定法(ELISA)是现有血清学检测工具的一项重大补充。过去两年将其应用于多种寄生虫病所获得的初步结果令人鼓舞,表明进一步研究和试验具有价值,这将有助于进行真正的评估。尽管该技术易于操作且相当灵敏,但在广泛应用之前仍有一些问题需要解决。本备忘录给出了技术细节,并讨论了该方法的优缺点。还对当前的应用和未来前景进行了综述。

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本文引用的文献

1
Immunoassay using antigen-enzyme conjugates.使用抗原-酶偶联物的免疫测定法。
FEBS Lett. 1971 Jun 24;15(3):232-236. doi: 10.1016/0014-5793(71)80319-8.

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