一种培养和测量人蜕膜毛细血管内皮细胞的新型可靠方法。

A new reliable method of culturing and measuring human decidual capillary endothelial cells.

作者信息

Peek M J, Fraser I S, Johannisson E, Markham R

机构信息

Department of Obstetrics and Gynaecology, University of Sydney, New South Wales, Australia.

出版信息

Exp Toxicol Pathol. 1994 Jul;46(2):149-54. doi: 10.1016/S0940-2993(11)80047-3.

DOI:10.1016/S0940-2993(11)80047-3

PMID:7987073

Abstract

A reliable method of culturing human decidual capillary endothelial cells was developed. Endothelial growth cell supplement, heparin and newborn bovine serum were added to the culture medium to facilitate growth. Decidual capillary endothelial cell cultures showed similar growth curves to the more traditional human umbilical cord vein endothelial cell cultures. Culture growth was assessed by a new morphometric method of measuring cell culture area with the use of a computerised measuring tablet. This new method was compared with the more laborious method of counting individual cells and was found to be as reliable and more efficient.

摘要

开发出了一种可靠的培养人蜕膜毛细血管内皮细胞的方法。向培养基中添加内皮生长细胞补充剂、肝素和新生牛血清以促进生长。蜕膜毛细血管内皮细胞培养物显示出与更传统的人脐静脉内皮细胞培养物相似的生长曲线。通过一种使用计算机测量平板测量细胞培养面积的新形态测量方法来评估培养生长情况。将这种新方法与更费力的逐个计数细胞的方法进行比较，发现它同样可靠且效率更高。