Katsumoto T, Higaki K, Ohno K, Onodera K
Laboratory of Electron Microscopy, Faculty of Medicine, Tottori University, Yonago, Japan.
Biol Cell. 1994;81(1):17-21. doi: 10.1016/0248-4900(94)90050-7.
The behavior of the primary cilia of 3Y1 cells in the interphase was investigated by indirect immunofluorescence microscopy and transmission electron microscopy, using an antibody for tubulin. At 4.5 h after scraping a part of a confluent cell sheet, the primary cilia of cells facing the wound were located predominantly forward of the nucleus on the wounded side, and were oriented in the direction of the leading lamellae. Cytoplasmic microtubules (MTs), emanating from around the base of the cilia, were well developed in the leading lamellae on the wounded side. On the other hand, in the cells of an unperturbed area away from the wounded edge, the primary cilia remained randomly distributed near the nucleus. The position and a certain well-defined orientation of a pair of centrioles seem to play an important role for the development of cytoplasmic MTs, and consequently the orientation of the centrioles is controlled by the primary cilia.
使用抗微管蛋白抗体，通过间接免疫荧光显微镜和透射电子显微镜研究了3Y1细胞在间期初级纤毛的行为。在刮除汇合细胞片的一部分4.5小时后，伤口侧面向伤口的细胞的初级纤毛主要位于细胞核前方的受伤侧，并沿前缘方向定向。从纤毛基部周围发出的细胞质微管（MTs）在受伤侧的前缘发育良好。另一方面，在远离受伤边缘的未受干扰区域的细胞中，初级纤毛仍随机分布在细胞核附近。一对中心粒的位置和特定的明确方向似乎对细胞质MTs的发育起重要作用，因此中心粒的方向由初级纤毛控制。
