Kussak A, Andersson B, Andersson K
National Institute of Occupational Health, Analytical Chemistry Division, Umeå, Sweden.
J Chromatogr B Biomed Appl. 1994 Jun 17;656(2):329-34. doi: 10.1016/0378-4347(94)00123-5.
A liquid chromatographic system with an automated clean-up procedure for aflatoxin Q1 in human urine is described. The samples were cleaned up by using immunoaffinity columns originally designed for aflatoxin M1. The chromatographic system was a C18 column with an acidic mobile phase of acetonitrile-water containing potassium bromide. Fluorescence detection (365/440 nm) of aflatoxin Q1 was enhanced by addition of bromine, using post-column derivatization, which was studied by factorial designs. Average recovery of aflatoxin Q1 in spiked 10-ml urine samples was 88% (R.S.D. = 6.4%) at a level of 50 pg/ml. The determination limit was 49.5 pg/ml urine.
