免疫亲和柱净化用于高效液相色谱法测定尿液中的黄曲霉毒素B1、B2、G1、G2、M1和Q1。

Immunoaffinity column clean-up for the high-performance liquid chromatographic determination of aflatoxins B1, B2, G1, G2, M1 and Q1 in urine.

作者信息

Kussak A, Andersson B, Andersson K

机构信息

National Institute of Occupational Health, Analytical Chemistry Division, Umeå, Sweden.

出版信息

J Chromatogr B Biomed Appl. 1995 Oct 20;672(2):253-9. doi: 10.1016/0378-4347(95)00227-a.

DOI:10.1016/0378-4347(95)00227-a

PMID:8581131

Abstract

A method for the determination of aflatoxins B1, B2, G1, G2, M1 and Q1 in human urine has been developed. The 10-ml urine samples were automatically cleaned up on immunoaffinity columns and analysed by high-performance liquid chromatography (HPLC), including post-column derivatization with bromine and fluorescence detection. Average aflatoxin recoveries were: B1 103%, B2 106%, G1 98% and G2 96% in the range 6.8-73 pg/ml of urine and M1 103% and Q1 100% in the range 18-97 pg/ml of urine. The relative standard deviations were all between 1% and 21%. The determination limits of aflatoxins in urine were 6.8 pg/ml for B1, B2, G1 and G2 and 18 pg/ml for M1 and Q1.

摘要

已开发出一种测定人尿中黄曲霉毒素B1、B2、G1、G2、M1和Q1的方法。10毫升尿样在免疫亲和柱上自动净化，然后用高效液相色谱法（HPLC）分析，包括柱后溴衍生化和荧光检测。黄曲霉毒素的平均回收率为：尿中6.8 - 73 pg/ml范围内，B1为103%，B2为106%，G1为98%，G2为96%；尿中18 - 97 pg/ml范围内，M1为103%，Q1为100%。相对标准偏差均在1%至21%之间。尿中黄曲霉毒素的测定限为：B1、B2、G1和G2为6.8 pg/ml，M1和Q1为18 pg/ml。