Peulve P, Laquerriere A, Hemet J, Tadie M
Experimental Neurosurgery Laboratory, UER de Medecine, Saint Etienne du Rouvray, France.
Brain Res. 1994 Aug 22;654(2):319-23. doi: 10.1016/0006-8993(94)90494-4.
Basic fibroblast growth factor (b-FGF) and alpha melanocyte stimulating hormone (alpha-MSH) were tested for their ability to promote axonal elongation on E14 fetal rat spinal cord cell culture, and to support cell survival. A similar development of neurite was observed in alpha-MSH treated cultures or in control cultures, with an axonal length ranging from 87.50 microns to 195.60 microns on day 3. Complete cell death occurred after 6 days of incubation. Whatever the concentration of b-FGF used (0.312-2.5 ng/ml), a significant increase (1.2- to 1.4-fold) in neurite length was observed, with neuronal survival up to 9 days.
在E14胎鼠脊髓细胞培养中，检测了碱性成纤维细胞生长因子（b-FGF）和α-黑素细胞刺激素（α-MSH）促进轴突伸长以及支持细胞存活的能力。在α-MSH处理的培养物或对照培养物中观察到了类似的神经突发育情况，第3天时轴突长度在87.50微米至195.60微米之间。孵育6天后细胞完全死亡。无论使用何种浓度的b-FGF（0.312 - 2.5纳克/毫升），神经突长度均显著增加（1.2至1.4倍），神经元存活可达9天。
