Papaspyrou M, Feinendegen L E, Müller-Gärtner H W
Institute for Medicine, Research Centre Jülich, Germany.
Cancer Res. 1994 Dec 15;54(24):6311-4.
To improve the effectiveness of boron neutron capture therapy, the possibility of stimulating boron uptake was investigated in an experimental model. B16F1 mouse melanoma cells were exposed to boronophenylalanine (BPA). The intracellular boron concentration followed Michaelis-Menten kinetics in the early incubation phase. In the late phase, cellular boron concentration was linearly related to the BPA concentration in the culture medium. Incubation with L-tyrosine before exposure to BPA (preloading) increased the intracellular boron concentration by a factor of three. It is concluded that in B16F1 cells BPA is transported by L and presumably ASC (alanine, serine, and cysteine) transport systems, and that boron uptake can be effectively stimulated by L-tyrosine preloading.
为提高硼中子俘获疗法的有效性，在一个实验模型中研究了刺激硼摄取的可能性。将B16F1小鼠黑色素瘤细胞暴露于硼苯丙氨酸（BPA）。在孵育早期，细胞内硼浓度遵循米氏动力学。在后期，细胞硼浓度与培养基中的BPA浓度呈线性相关。在暴露于BPA之前用L-酪氨酸孵育（预加载）可使细胞内硼浓度增加三倍。得出的结论是，在B16F1细胞中，BPA通过L转运系统以及推测的ASC（丙氨酸、丝氨酸和半胱氨酸）转运系统进行转运，并且L-酪氨酸预加载可有效刺激硼摄取。
