Basha M A, Gross K B, Gwizdala C J, Haidar A H, Popovich J
Department of Internal Medicine, Henry Ford Health Sciences Center, Detroit, Mich.
Chest. 1994 Dec;106(6):1757-65. doi: 10.1378/chest.106.6.1757.
To characterize the pulmonary response of asthmatic and healthy nonsmoking adult men to 0.20 ppm ozone by controlled chamber exposure.
A prospective, crossover study of five atopic asthmatic and five normal subjects randomly exposed to ozone and filtered purified air (FPA) for 6 h, consisting of 30-min alternating periods of rest and moderate exercise. The two exposures were separated by at least 30 days.
A controlled exposure in a stainless steel chamber.
Five atopic asthmatic and five normal subjects between 18 and 45 years of age. Treatment with medications was withheld from asthmatics prior to the exposures. All subjects were nonsmokers.
Symptoms were assessed throughout the exposures. Pulmonary function was measured at baseline, hourly throughout an exposure, and after an exposure. Bronchoalveolar lavage (BAL) was performed 18 h after the completion of an exposure. The BAL fluid (BALF) was analyzed for cell count and differential; the cell-free supernatant was analyzed for albumin, tumor necrosis factor (TNF), interleukin 1 (IL-1), interleukin 6 (IL-6), and interleukin 8 (IL-8).
There were statistically significant increases in IL-8 levels, as well as percent polymorphonuclear neutrophils (PMNs) and PMNs per milliliter of lavage in asthmatics exposed to ozone as compared with the same asthmatics exposed to FPA and the same normal subjects exposed to ozone and FPA. Interleukin 6 was also significantly increased in asthmatics exposed to ozone. The BALF albumin, TNF, and IL-1 levels were not significantly different among the four groups. There were no differences between asthmatics and healthy controls exposed to ozone or FPA in baseline to postexposure FEV1, FVC, FEV1/FVC, and sRaw.
We conclude that asthmatics exposed to ozone develop a significant BALF neutrophilia and increased levels of the cytokines, IL-8 and IL-6. These BALF findings occur even though the level of ozone exposure was not significant enough to reduce pulmonary function.
通过控制室内暴露，描述哮喘患者和健康非吸烟成年男性对0.20 ppm臭氧的肺部反应。
一项前瞻性交叉研究，五名特应性哮喘患者和五名正常受试者随机暴露于臭氧和过滤净化空气（FPA）中6小时，包括30分钟交替的休息和适度运动时段。两次暴露间隔至少30天。
在不锈钢室内进行控制暴露。
五名18至45岁的特应性哮喘患者和五名正常受试者。哮喘患者在暴露前停用药物治疗。所有受试者均不吸烟。
在整个暴露过程中评估症状。在基线、暴露期间每小时以及暴露后测量肺功能。暴露完成后18小时进行支气管肺泡灌洗（BAL）。分析BAL液（BALF）的细胞计数和分类；分析无细胞上清液中的白蛋白、肿瘤坏死因子（TNF）、白细胞介素1（IL-1）、白细胞介素6（IL-6）和白细胞介素8（IL-8）。
与暴露于FPA的相同哮喘患者以及暴露于臭氧和FPA的相同正常受试者相比，暴露于臭氧的哮喘患者的IL-8水平、多形核中性粒细胞（PMN）百分比和每毫升灌洗液中的PMN有统计学显著增加。暴露于臭氧的哮喘患者的白细胞介素6也显著增加。四组之间的BALF白蛋白、TNF和IL-1水平无显著差异。暴露于臭氧或FPA的哮喘患者与健康对照在基线至暴露后FEV1、FVC、FEV1/FVC和sRaw方面无差异。
我们得出结论，暴露于臭氧的哮喘患者会出现显著的BALF中性粒细胞增多以及细胞因子IL-8和IL-6水平升高。即使臭氧暴露水平不足以降低肺功能，这些BALF结果仍然出现。
