在低温下，D1-D2-细胞色素b559复合物中不可逆的光诱导形成P680 +和还原型细胞色素b559。

Irreversible light-induced formation of P680+ and reduced cytochrome b559 in the D1-D2-Cyt b-559 complex at low temperature.

作者信息

Kaminskaya O P, Shuvalov V A

机构信息

Institute of Soil Sciences and Photosynthesis, Russian Academy of Sciences, Pushchino, Moscow region.

出版信息

FEBS Lett. 1994 Dec 5;355(3):301-4. doi: 10.1016/0014-5793(94)01122-2.

DOI:10.1016/0014-5793(94)01122-2

PMID:7988693

Abstract

Cytochrome b559 in D1-D2-Cyt b-559 complexes from spinach can be photoreduced in the presence of DBMIB at a temperature of 180-240 K upon continuous illumination. The reduction of Cyt b-559 is accompanied by oxidation of P680. At 240 K recombination of P680+ and reduced Cyt b-559 is complete in several seconds. At 220 K and below, the state P680+Cyt b-559red can be trapped for a long time. This indicates that the photoreduced heme is incapable of electron transfer to P680+ at 220 K and below. On the other hand, the chemically reduced heme of Cyt b-559 is oxidized by P680+ at 77 K. These results are consistent with the presence of two kinds of Cyt b-559 hemes in D1-D2-Cyt b-559 complexes which participate in different ways in the photochemical reactions.

摘要

在持续光照下，菠菜的D1-D2-细胞色素b-559复合物中的细胞色素b559在180 - 240 K的温度下，在DBMIB存在的情况下可被光还原。细胞色素b-559的还原伴随着P680的氧化。在240 K时，P680+与还原态细胞色素b-559的重组在几秒钟内完成。在220 K及以下，P680+Cyt b-559red状态可被长时间捕获。这表明在220 K及以下，光还原的血红素无法将电子转移至P680+。另一方面，在77 K时，细胞色素b-559的化学还原血红素被P680+氧化。这些结果与D1-D2-细胞色素b-559复合物中存在两种以不同方式参与光化学反应的细胞色素b-559血红素一致。