Kikkawa R, Haneda M, Uzu T, Koya D, Sugimoto T, Shigeta Y
Third Department of Medicine, Shiga University of Medical Science, Japan.
Diabetologia. 1994 Aug;37(8):838-41. doi: 10.1007/BF00404342.
The activities and expression of protein kinase C isoenzymes were examined in glomerular mesangial cells cultured under high glucose conditions. Exposure of cells to high glucose concentrations (27.8 mmol/l) for more than 3 days resulted in a significant elevation of protein kinase C activities in the membrane fraction. Of the protein kinase C isoenzymes, the levels of protein kinase C alpha significantly increased in the membrane fraction after 3 days of exposure to glucose, and protein kinase C zeta increased after 5 days of exposure. Levels of protein kinase C delta and epsilon remained unchanged and protein kinase, C beta and gamma were not detected. These results indicate that protein kinase C alpha and zeta are translocated under high glucose conditions possibly through different mechanisms.
在高糖条件下培养的肾小球系膜细胞中检测了蛋白激酶C同工酶的活性和表达。细胞暴露于高糖浓度（27.8 mmol/l）超过3天导致膜组分中蛋白激酶C活性显著升高。在蛋白激酶C同工酶中，暴露于葡萄糖3天后膜组分中蛋白激酶Cα的水平显著增加，暴露5天后蛋白激酶Cζ增加。蛋白激酶Cδ和ε的水平保持不变，未检测到蛋白激酶Cβ和γ。这些结果表明，蛋白激酶Cα和ζ在高糖条件下可能通过不同机制发生易位。
