Hayashi T, Matsumoto H, Ohnishi M, Yokota S, Shinomiya T, Kageyama M, Terawaki Y
Department of Bacteriology, Shinshu University School of Medicine, Matsumoto, Japan.
FEMS Microbiol Lett. 1994 Oct 1;122(3):239-44. doi: 10.1111/j.1574-6968.1994.tb07174.x.
phi CTX is a temperate phage of Pseudomonas aeruginosa harbouring the ctx gene that encodes cytotoxin (CTX). We identified phi CTX as an R pyocin-related phage, by serological and molecular analysis, based on the findings that the infectivity of the phage was inhibited with the antisera directed R pyocins and R pyocin-related phages and that the phi CTX genome showed DNA homology to the genome of PS17 (a representative of the R pyocin-related phages) as well as to the pyocin R2 genes. Another new CTX-converting, R pyocin-related phage named PS21 was isolated from a CTX-producing strain of P. aeruginosa, suggesting the distribution of the ctx gene by certain members of R pyocin-related phage family.
噬菌体CTX是铜绿假单胞菌的一种温和噬菌体，携带编码细胞毒素（CTX）的ctx基因。基于噬菌体的感染性被针对R型绿脓菌素和R型绿脓菌素相关噬菌体的抗血清所抑制，以及噬菌体CTX基因组与PS17（R型绿脓菌素相关噬菌体的代表）基因组以及绿脓菌素R2基因显示出DNA同源性这一发现，我们通过血清学和分子分析将噬菌体CTX鉴定为一种与R型绿脓菌素相关的噬菌体。另一种新的能转化CTX的、与R型绿脓菌素相关的噬菌体PS21是从一株产CTX的铜绿假单胞菌菌株中分离出来的，这表明ctx基因在R型绿脓菌素相关噬菌体家族的某些成员中存在分布。
