长枝木霉egl1基因的转录调控

Transcriptional regulation of the Trichoderma longibrachiatum egl1 gene.

作者信息

González R, Pérez-González J A, González-Candelas L, Ramón D

机构信息

Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de los Alimentos (CSIC), Valencia, Spain.

出版信息

FEMS Microbiol Lett. 1994 Oct 1;122(3):303-7. doi: 10.1111/j.1574-6968.1994.tb07184.x.

DOI:10.1111/j.1574-6968.1994.tb07184.x

PMID:7988872

Abstract

Transcription of the Trichoderma longibrachiatum egl1 gene is induced in the presence of lactose and beta-methylglucoside and repressed by glucose. A DNA fragment containing 722 bp upstream of the ATG codon has been sequenced. The gene has two major transcription start points (20 and 24 nucleotides upstream from the ATG codon) and several transcription termination points (located in a region around 130 nt downstream of the stop codon). Two 6-mer sequences (5'-CTGGAG-3') separated by 16 bp are present in the egl1 gene promoter. These sequences match the Aspergillus nidulans consensus CreA binding site and might be implicated in carbon catabolite repression of egl1 transcription.

摘要

长枝木霉egl1基因的转录在乳糖和β-甲基葡萄糖苷存在时被诱导，而在葡萄糖存在时被抑制。已对包含ATG密码子上游722 bp的DNA片段进行了测序。该基因有两个主要转录起始点（位于ATG密码子上游20和24个核苷酸处）和几个转录终止点（位于终止密码子下游约130 nt的区域）。egl1基因启动子中存在两个由16 bp隔开的6聚体序列（5'-CTGGAG-3'）。这些序列与构巢曲霉共有CreA结合位点匹配，可能与egl1转录的碳代谢物阻遏有关。