Dix B R, Robbins P, Soong R, Jenner D, House A K, Iacopetta B J
Department of Surgery, University of Western Australia, Nedlands.
Int J Cancer. 1994 Dec 15;59(6):747-51. doi: 10.1002/ijc.2910590606.
Our study was undertaken to determine the prognostic significance of several common genetic alterations observed in colorectal carcinomas. We have previously analysed loss of heterozygosity of the MCC, APC, p53 and DCC tumour suppressor gene loci as well as p53 gene mutations and protein over-expression in a series of 100 Dukes' stage B and C colorectal tumours obtained at surgery. To extend our observations of alterations that may occur in these tumours, mutations to the c-Ki-ras oncogene and APC tumour suppressor gene were detected by PCR single-strand conformation polymorphism analysis. Short-term follow-up revealed no significant association between overall patient survival and any single, or combination of, genetic alteration(s). Surprisingly, patients whose tumours showed evidence of p53 protein over-expression/accumulation by immunocytochemistry (ICC) had a significantly better prognosis (p = 0.039) than those whose tumours had no p53 ICC reactivity.
我们开展这项研究是为了确定在结直肠癌中观察到的几种常见基因改变的预后意义。我们之前分析了100例手术切除的杜克B期和C期结直肠癌肿瘤中MCC、APC、p53和DCC肿瘤抑制基因位点的杂合性缺失，以及p53基因突变和蛋白过表达情况。为了扩展我们对这些肿瘤可能发生的改变的观察，通过聚合酶链反应单链构象多态性分析检测了c-Ki-ras癌基因和APC肿瘤抑制基因的突变。短期随访显示，总体患者生存率与任何单一基因改变或基因改变组合之间均无显著关联。令人惊讶的是，通过免疫细胞化学（ICC）检测显示肿瘤有p53蛋白过表达/积累证据的患者，其预后（p = 0.039）明显优于肿瘤无p53 ICC反应性的患者。
