Fujii Y, Imamura M, Han M, Hashino S, Zhu X, Kobayashi H, Imai K, Kasai M, Sakurada K, Miyazaki T
3rd Department of Internal Medicine, Hokkaido University School of Medicine, Japan.
Int J Immunopharmacol. 1994 Aug;16(8):615-22. doi: 10.1016/0192-0561(94)90134-1.
Ren-shen-yang-rong-tang (Japanese name: Ninjin-youei-to, NYT), a traditional Chinese herbal medicine, was evaluated for recipient-mediated effect on hematopoietic recovery in a murine model of syngeneic bone marrow transplantation (BMT). BALB/c recipient mice were preconditioned with a lethal total body irradiation (TBI) at a dose of 6.5 Gy and transplanted with syngeneic bone marrow (BM) cells. NYT treatments, given intraperitoneally (i.p.) once per day for 3 consecutive days in a dose of 0.625 mg, were performed either before or after TBI and BMT to assess any recipient-mediated effect of this compound. NYT pretreatment was as effective as NYT posttreatment in enhancing the total number of colony-forming unit erythroid (CFU-E) and colony-forming unit granulocyte-macrophage (CFU-GM) per marrow and spleen after TBI and BMT. NYT pretreatment caused a significant increase in marrow and splenic CFU-E and CFU-GM numbers over a prolonged period following TBI and BMT, and affected late-stage erythropoiesis (CFU-E) more profoundly than early-stage erythropoiesis (burst-forming unit erythroid, BFU-E). NYT pretreatment significantly accelerate recovery of not only erythrocyte and leukocyte counts but also platelet counts after transplantation with a limited number (1 x 10(5)) of BM cells. The same treatment, however, was significantly less effective in hematopoietic recovery after transplantation with a minimal number (1 x 10(4)) of BM cells, indicating that NYT accelerates recovery of donor-derived rather than recipient-derived cells.(ABSTRACT TRUNCATED AT 250 WORDS)
人参养荣汤（日本名称：人参养荣汤，NYT），一种传统的中草药，在同基因骨髓移植（BMT）的小鼠模型中，对其受体介导的造血恢复作用进行了评估。将BALB/c受体小鼠用6.5 Gy剂量的致死性全身照射（TBI）进行预处理，然后移植同基因骨髓（BM）细胞。NYT治疗，以0.625 mg的剂量连续3天每天腹腔内（i.p.）给药一次，在TBI和BMT之前或之后进行，以评估该化合物的任何受体介导的作用。NYT预处理在增强TBI和BMT后每个骨髓和脾脏的红系集落形成单位（CFU-E）和粒-巨噬细胞集落形成单位（CFU-GM）总数方面与NYT后处理一样有效。NYT预处理在TBI和BMT后的较长时间内导致骨髓和脾脏CFU-E和CFU-GM数量显著增加，并且对晚期红细胞生成（CFU-E）的影响比对早期红细胞生成（红系爆式集落形成单位，BFU-E）更深刻。NYT预处理在移植有限数量（1×10⁵）的BM细胞后不仅显著加速了红细胞和白细胞计数的恢复，而且还加速了血小板计数的恢复。然而，相同的处理在移植最少数量（1×10⁴）的BM细胞后的造血恢复方面效果明显较差，表明NYT加速了供体来源而非受体来源细胞的恢复。（摘要截短于250字）
