De Gandarias J M, Irazusta J, Fernandez D, Varona A, Casis L
Department of Physiology, Medical School, University of the Basque Country, Bilbao, Spain.
Int J Neurosci. 1994 Jul;77(1-2):53-60. doi: 10.3109/00207459408986018.
In this work, we have described decreases of pGlu-peptidase I activity, detected using pGlu-beta-naphtylamide as substrate, from 9 to the 20 days after birth in five brain areas. In addition, we have found sexual differences in the enzyme activity in the hypothalamic-pituitary axis in all ages investigated. pGlu-peptidase cleaves the pGlu-aminoacid bond of several neuropeptides such as thyroliberin, luliberin and neurotensin. The decrease in the activity of pGlu-peptidase coincidents with the increases observed in brain thyroliberin concentration and the decreases in His-Pro diketopiperazine. It is suggested that this enzyme could play a part in the normal development of the rat brain.
在这项研究中，我们描述了以对谷氨酰胺-β-萘酰胺为底物检测到的，出生后9至20天内五个脑区中对谷氨酰胺肽酶I活性的降低。此外，我们发现在所有研究年龄的下丘脑-垂体轴中，该酶活性存在性别差异。对谷氨酰胺肽酶可裂解几种神经肽的对谷氨酰胺-氨基酸键，如促甲状腺素释放激素、促黄体生成素释放激素和神经降压素。对谷氨酰胺肽酶活性的降低与脑促甲状腺素释放激素浓度的升高以及组氨酸-脯氨酸二酮哌嗪的降低相一致。提示该酶可能在大鼠脑的正常发育中起作用。
