从猫身上分离出的卟啉单胞菌属过氧化氢酶的特性分析

Characterization of the catalase of the genus Porphyromonas isolated from cats.

作者信息

Love D N, Redwin J

机构信息

Department of Veterinary Pathology, University of Sydney, NSW, Australia.

出版信息

J Appl Bacteriol. 1994 Oct;77(4):421-5. doi: 10.1111/j.1365-2672.1994.tb03444.x.

DOI:10.1111/j.1365-2672.1994.tb03444.x

PMID:7989270

Abstract

Crude cell extracts from members of the genus Porphyromonas isolated from cats were examined in SDS-PAGE and nondenaturing PAGE. In each of the species catalase activity was detected as a single band with characteristics of typical bacterial catalases, i.e. each catalase functioned over a broad pH range (pH 5-10), was not inhibited by chloroform-ethanol, did not possess detectable peroxidase activity, and was irreversibly inhibited by 3-amino-1,2,4 triazole. The catalase enzyme of P. gingivalis VPB 3492, P. circumdentaria NCTC 12469, P. salivosa VPB 3313 and VPB 3444 was inactivated at 71, 66.5, 63.5 and 57 degrees C respectively. The molecular weights of the enzymes from P. gingivalis VPB 3492, P. circumdentaria NCTC 12469, P. salivosa NCTC 11632 and P. salivosa VPB 3444 were 200,000, 216,000, 209,000 and 200,000 Da respectively.

摘要

对从猫身上分离出的卟啉单胞菌属成员的粗细胞提取物进行了十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和非变性聚丙烯酰胺凝胶电泳（nondenaturing PAGE）检测。在每个物种中，过氧化氢酶活性均被检测为具有典型细菌过氧化氢酶特征的单一条带，即每种过氧化氢酶在较宽的pH范围（pH 5-10）内发挥作用，不受氯仿-乙醇抑制，不具有可检测到的过氧化物酶活性，且被3-氨基-1,2,4-三唑不可逆地抑制。牙龈卟啉单胞菌VPB 3492、齿周卟啉单胞菌NCTC 12469、涎腺卟啉单胞菌VPB 3313和VPB 3444的过氧化氢酶分别在71、66.5、63.5和57摄氏度失活。牙龈卟啉单胞菌VPB 3492、齿周卟啉单胞菌NCTC 12469、涎腺卟啉单胞菌NCTC 11632和涎腺卟啉单胞菌VPB 3444的酶的分子量分别为200,000、216,000、209,000和200,000道尔顿。