牙周健康和疾病期间猫牙龈边缘三种卟啉单胞菌属猫源物种之间的关联。

Associations amongst three feline Porphyromonas species from the gingival margin of cats during periodontal health and disease.

作者信息

Norris J M, Love D N

机构信息

Department of Veterinary Anatomy and Pathology, University of Sydney, NSW, Australia.

出版信息

Vet Microbiol. 1999 Mar 12;65(3):195-207. doi: 10.1016/s0378-1135(98)00290-9.

DOI:10.1016/s0378-1135(98)00290-9

PMID:10189194

Abstract

Digoxigenin labelled whole chromosomal DNA probes directed against three feline members of the genus Porphyromonas (P. gingivalis VPB 3492, P. circumdentaria NCTC 12469T and P. salivosa VPB 3313) were used to identify and quantify organisms in samples taken from the gingival margins of 40 domestic cats with different grades of periodontal disease. At the right upper canine tooth, the grade of periodontal disease ranged from 0 to 5 and the cfu of facultative/obligate anaerobes ranged from 5.5 x 10(4) to 2.0 x 10(6)). In 38 of the 40 cats, at least one of the three Porphyromonas species was isolated and regression analysis showed that the cfu of total Porphyromonas sp. was a highly significant indicator of the grade of periodontal disease (p < 0.001, R2 0.510). Feline P. gingivalis was isolated from 37 of the 40 cats and regression analysis showed that it was a highly significant predictor of the grade of periodontal disease (p < 0.001, R2 0.561). The cfu of P. salivosa was a significant predictor of the grade of periodontal disease (p < 0.001, R2 0.286) and regression analysis showed that there was a significant positive relationship between cfu of P. circumdentaria and grade of periodontal disease (p = 0.018, R2 0.116). The periodontal grades at the right upper third premolar tooth ranged from 0 to 6. The cfu of facultative/obligate anaerobes isolated ranged from 1.2 x 10(5) to 7.9 x 10(6), and regression analysis showed that cfu was a significant predictor of periodontal grade (p < 0.001, R2 0.378). The cfu of total Porphyromonas species ranged from 1.2 x 10(4) to 1.7 x 10(6) and regression analysis of the cfu against the grade of periodontal disease showed a highly significant association (p < 0.001, R2 0.633). The cfu of P. gingivalis ranged from 0 to 1.1 x 10(6) and regression analysis of the cfu of P. gingivalis against the grade of periodontal disease showed a highly significant association (p < 0.001, R2 0.439). The cfu of P. salivosa was a significant predictor of the grade of periodontal disease (p < 0.001, R2 0.479) and the same association was found between cfu of P. circumdentaria and grade of periodontal disease (p = 0.002, R2 0.204). This study has established Porphyromonas as anumerically significant and highly prevalent genus in feline periodontal disease.

摘要

针对卟啉单胞菌属的三个猫源菌型（牙龈卟啉单胞菌VPB 3492、齿周卟啉单胞菌NCTC 12469T和唾液卟啉单胞菌VPB 3313）的地高辛配体标记全染色体DNA探针，用于鉴定和定量取自40只患有不同程度牙周病的家猫牙龈边缘样本中的微生物。在右上犬齿处，牙周病程度从0到5，兼性/专性厌氧菌的菌落形成单位（cfu）范围为5.5×10⁴至2.0×10⁶。在40只猫中的38只中，分离出了三种卟啉单胞菌中的至少一种，回归分析表明，总卟啉单胞菌属的cfu是牙周病程度的高度显著指标（p<0.001，R² 0.510）。从40只猫中的37只分离出了猫牙龈卟啉单胞菌，回归分析表明它是牙周病程度的高度显著预测指标（p<0.001，R² 0.561）。唾液卟啉单胞菌的cfu是牙周病程度的显著预测指标（p<0.001，R² 0.286），回归分析表明齿周卟啉单胞菌的cfu与牙周病程度之间存在显著正相关（p = 0.018，R² 0.116）。右上第三前磨牙处的牙周病程度范围为0到6。分离出的兼性/专性厌氧菌的cfu范围为1.2×10⁵至7.9×10⁶，回归分析表明cfu是牙周病程度的显著预测指标（p<0.001，R² 0.378）。总卟啉单胞菌属的cfu范围为1.2×10⁴至1.7×10⁶，cfu与牙周病程度的回归分析显示出高度显著的相关性（p<0.001，R² 0.633）。牙龈卟啉单胞菌的cfu范围为0至1.1×10⁶，牙龈卟啉单胞菌的cfu与牙周病程度的回归分析显示出高度显著的相关性（p<0.001，R² 0.439）。唾液卟啉单胞菌的cfu是牙周病程度的显著预测指标（p<0.001，R² 0.479），并且在齿周卟啉单胞菌的cfu与牙周病程度之间发现了相同的相关性（p = 0.002，R² 0.204）。本研究已确定卟啉单胞菌属在猫牙周病中是数量上具有重要意义且高度普遍的菌属。