使用单克隆抗体在体外和体内鉴定巨细胞病毒感染的细胞。

Identification of GPCMV infected cells in vitro and in vivo with a monoclonal antibody.

作者信息

Jones C T, Keay S K, Swoveland P T

机构信息

Department of Pathology, University of Maryland, School of Medicine, Baltimore 21201.

出版信息

J Virol Methods. 1994 Jul;48(2-3):133-44. doi: 10.1016/0166-0934(94)90113-9.

DOI:10.1016/0166-0934(94)90113-9

PMID:7989431

Abstract

A monoclonal antibody was made which identifies a 160-180 kDa structural protein in guinea pig cytomegalovirus (GPCMV) infected cells by Western blot using non-reducing conditions. This protein was shown to be a virion structural protein by purification of GPCMV on a density viscosity gradient and Western blot analysis. Phosphoanacetic acid (PAA) experiments suggest that the protein is a late GPCMV protein. In vitro the monoclonal antibody labels a cytoplasmic protein in infected guinea pig embryo fibroblasts by 12 h postinfection. The monoclonal antibody also identifies GPCMV infected cells in vivo in paraffin embedded formalin fixed tissue.

摘要

制备了一种单克隆抗体，该抗体在非还原条件下通过蛋白质免疫印迹法，可识别豚鼠巨细胞病毒（GPCMV）感染细胞中的一种160 - 180 kDa结构蛋白。通过在密度粘度梯度上纯化GPCMV并进行蛋白质免疫印迹分析，表明该蛋白是一种病毒粒子结构蛋白。磷乙酸（PAA）实验表明该蛋白是一种GPCMV晚期蛋白。在体外，感染后12小时，该单克隆抗体标记感染的豚鼠胚胎成纤维细胞中的一种细胞质蛋白。该单克隆抗体还可在体内石蜡包埋的福尔马林固定组织中识别GPCMV感染的细胞。