Bremer C W, du Plessis D H, van Dijk A A
Biochemistry Division, Onderstepoort Veterinary Institute, Republic of South Africa.
J Virol Methods. 1994 Jul;48(2-3):245-56. doi: 10.1016/0166-0934(94)90123-6.
Non-structural protein NS2 and core protein VP7 of African horsesickness virus serotype 3 (AHSV3) were expressed in Spodoptera frugiperda cells by recombinant baculoviruses containing the relevant genes. These proteins were purified and analysed by polyacrylamide gel electrophoresis and Western blot. NS2 and VP7 were used separately as antigens in an indirect ELISA for the detection of AHSV antibodies. Both antigens cross-reacted with hyperimmune guinea-pig antisera to infected cell lysates of all nine known AHSV serotypes and to antisera obtained from horses immunized with attenuated virus of seven AHSV serotypes.
通过含有相关基因的重组杆状病毒，在草地贪夜蛾细胞中表达了非洲马瘟病毒3型（AHSV3）的非结构蛋白NS2和核心蛋白VP7。对这些蛋白进行纯化，并通过聚丙烯酰胺凝胶电泳和蛋白质免疫印迹法进行分析。NS2和VP7分别用作间接ELISA中的抗原，用于检测AHSV抗体。两种抗原均与针对所有九种已知AHSV血清型感染细胞裂解物的超免疫豚鼠抗血清以及从用七种AHSV血清型减毒病毒免疫的马匹获得的抗血清发生交叉反应。
