Vićovac L M, Starkey P M, Aplin J D
INEP, University of Belgrade, Zemun, Yugoslavia.
J Clin Endocrinol Metab. 1994 Dec;79(6):1877-82. doi: 10.1210/jcem.79.6.7989496.
Human decidua contains resident decidual cells alongside a population of bone marrow-derived cells, among which macrophages and large granular lymphocytes are most abundant. We hypothesized that soluble effectors produced by bone marrow-derived cells may modulate the function of the decidual cells. To investigate this, a cell purification protocol was devised that involved digestion of first-trimester decidua with collagenase and hyaluronidase to produce a mixed stromal cell suspension from which the bone marrow-derived cells were removed using immunomagnetic beads coated with anti-CD45. The resulting stromal cells were maintained in culture in the presence of progesterone and were found to produce PRL. The effect of a panel of cytokines on PRL production was examined. Tumor necrosis factors-alpha and -beta had a dose-dependent inhibitory effect, and tumor necrosis factor receptors were identified on the cells. Interleukin 1 alpha and 1 beta, platelet-derived growth factor, and transforming growth factor-beta 1 were also found to inhibit PRL production, and platelet-derived growth factor and transforming growth factor-beta 1 stimulated cell proliferation. These findings suggest an interaction between the immune and endocrine systems in regulating the maternal environment of early pregnancy.
人蜕膜中除了一群骨髓来源的细胞外，还含有驻留蜕膜细胞，其中巨噬细胞和大颗粒淋巴细胞最为丰富。我们推测骨髓来源的细胞产生的可溶性效应分子可能会调节蜕膜细胞的功能。为了对此进行研究，我们设计了一种细胞纯化方案，该方案包括用胶原酶和透明质酸酶消化孕早期蜕膜，以产生混合的基质细胞悬液，然后使用包被抗CD45的免疫磁珠从该悬液中去除骨髓来源的细胞。将得到的基质细胞在孕酮存在的情况下进行培养，发现它们能产生催乳素。研究了一组细胞因子对催乳素产生的影响。肿瘤坏死因子-α和-β具有剂量依赖性抑制作用，并且在细胞上鉴定出了肿瘤坏死因子受体。白细胞介素1α和1β、血小板衍生生长因子以及转化生长因子-β1也被发现可抑制催乳素的产生，而血小板衍生生长因子和转化生长因子-β1则刺激细胞增殖。这些发现表明，在调节早孕母体环境方面，免疫和内分泌系统之间存在相互作用。
