Sawai K, Matsuzaki N, Okada T, Shimoya K, Koyama M, Azuma C, Saji F, Murata Y
Department of Obstetrics and Gynecology, Osaka University Medical School, Suita, Japan.
Biol Reprod. 1997 May;56(5):1274-80. doi: 10.1095/biolreprod56.5.1274.
The production of leukemia inhibitory factor (LIF) is suggested to be critical for the successful implantation of blastocysts into decidua, because LIF expression is essential for the implantation of mouse blastocytes. We investigated the regulation of LIF production by decidual cytokines and steroid hormones. Stimulation of decidual cells by interleukin-1, tumor necrosis factor alpha, or transforming growth factor beta augmented LIF production in a dose-dependent manner. Moreover, estradiol, a steroid hormone that increases during ovulation and early pregnancy, also enhanced LIF production in a dose-dependent manner. These responses were blocked by protein kinase C (PKC) inhibitor but not by other kinase inhibitors, suggesting an important role of PKC in decidual LIF production mediated by cytokines and estradiol. We also showed that stimulating decidual cells with LIF failed to stimulate DNA synthesis and prolactin production in these cells. In summary, LIF was mainly localized in the decidual glands and stroma, and its production was increased by cytokines and estradiol in a dose-dependent fashion; but stimulation of decidual cells by LIF did not influence their proliferation or their prolactin production.
白血病抑制因子（LIF）的产生被认为对胚泡成功植入蜕膜至关重要，因为LIF的表达对小鼠胚泡的植入必不可少。我们研究了蜕膜细胞因子和类固醇激素对LIF产生的调节作用。白细胞介素-1、肿瘤坏死因子α或转化生长因子β刺激蜕膜细胞可使LIF产生呈剂量依赖性增加。此外，在排卵和妊娠早期增加的类固醇激素雌二醇也以剂量依赖性方式增强了LIF的产生。这些反应被蛋白激酶C（PKC）抑制剂阻断，但未被其他激酶抑制剂阻断，这表明PKC在细胞因子和雌二醇介导的蜕膜LIF产生中起重要作用。我们还表明，用LIF刺激蜕膜细胞未能刺激这些细胞中的DNA合成和催乳素产生。总之，LIF主要定位于蜕膜腺和基质中，其产生通过细胞因子和雌二醇以剂量依赖性方式增加；但LIF刺激蜕膜细胞并不影响其增殖或催乳素产生。
