Gunn L K, Homa S T, Searle M J, Chard T
Department of Reproductive Physiology, St. Bartholomew's Hospital Medical College, London, UK.
Hum Reprod. 1994 Aug;9(8):1522-7. doi: 10.1093/oxfordjournals.humrep.a138742.
The aim of this study was to determine if the human pre-embryo produces a substance similar to the trophoblast interferon secreted by ruminant trophoblasts. Human embryos surplus to in-vitro fertilization (IVF) treatment were cultured up to 14 days following IVF. Viable cultures were determined by microscopic examination and by assay of the culture medium for human chorionic gonadotrophin (HCG). Four stages of development were visually identified: pre-blastocyst, unhatched, part-hatched and fully hatched blastocyst. HCG was detected in medium which had contained the more developmentally advanced embryos. A total of 62 samples were assayed for human interferon-alpha (IFN-alpha), including all cultures presumed viable. None contained detectable IFN-alpha immunoreactivity. Out of 14 candidate samples subjected to cytopathic effect reduction assay, none contained antiviral activity. We suggest that a trophoblast-derived interferon, unlike HCG, does not play a significant role in the maternal recognition of pregnancy in humans.
本研究的目的是确定人类早期胚胎是否会产生一种类似于反刍动物滋养层分泌的滋养层干扰素的物质。将体外受精（IVF）治疗后剩余的人类胚胎在IVF后培养长达14天。通过显微镜检查和检测培养基中的人绒毛膜促性腺激素（HCG）来确定培养物是否存活。通过视觉识别出四个发育阶段：囊胚前期、未孵化、部分孵化和完全孵化的囊胚。在含有发育更成熟胚胎的培养基中检测到了HCG。总共对62个样本进行了人α干扰素（IFN-α）检测，包括所有假定存活的培养物。没有一个样本含有可检测到的IFN-α免疫反应性。在接受细胞病变效应降低试验的14个候选样本中，没有一个具有抗病毒活性。我们认为，与HCG不同，滋养层来源的干扰素在人类母体对妊娠的识别中不起重要作用。
