Dokras A, Sargent I L, Ross C, Gardner R L, Barlow D H
Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Oxford, UK.
Hum Reprod. 1991 Sep;6(8):1143-51. doi: 10.1093/oxfordjournals.humrep.a137500.
Micromanipulation of human oocytes and embryos has provided new opportunities for both the treatment of infertility and the preimplantation diagnosis of genetic disease. It is important to determine whether manipulated embryos develop normally in vitro, as an indication of their suitability for transfer. However, at present there is little information on the development of non-manipulated embryos in vitro for comparison. We have therefore monitored morphological changes and human chorionic gonadotrophin (HCG) secretion in 36 non-manipulated human embryos, including 26 blastocysts and 10 cavitating morulae, daily from day 3 to day 14 of culture. Hatching was observed in 10 (38.5%) blastocysts and five of these adhered to the culture dish and appeared viable until day 14. The secretion of HCG was first detected on day 8, peaked at day 10 (51.11 +/- 8.7 mIU/ml) and then declined but was still detectable in four blastocysts on day 14. There was no overall difference in HCG secretion by hatched blastocysts and those which remained within the zona. However, those hatched blastocysts which showed adherence had significantly increased (P less than 0.05) HCG secretion. For individual blastocysts, the pattern of HCG secretion correlated well with the assessment of morphology. These data provide the basis for comparative studies of morphological changes and HCG secretion in manipulated embryos.
对人类卵母细胞和胚胎进行显微操作，为不孕症治疗和遗传病植入前诊断带来了新机遇。确定经操作的胚胎在体外能否正常发育，对于判断其是否适合移植至关重要。然而，目前关于未操作胚胎在体外发育情况的可用于比较的信息很少。因此，我们对36个未操作的人类胚胎（包括26个囊胚和10个正在形成腔隙的桑葚胚）从培养第3天到第14天每天监测其形态变化和人绒毛膜促性腺激素（HCG）分泌情况。在10个（38.5%）囊胚中观察到孵化，其中5个附着在培养皿上，直到第14天看起来仍有活力。HCG分泌在第8天首次检测到，在第10天达到峰值（51.11±8.7 mIU/ml），然后下降，但在第14天仍有4个囊胚可检测到。孵化的囊胚和仍在透明带内的囊胚之间的HCG分泌没有总体差异。然而，那些附着的孵化囊胚的HCG分泌显著增加（P<0.05）。对于单个囊胚，HCG分泌模式与形态学评估密切相关。这些数据为比较经操作胚胎的形态变化和HCG分泌提供了基础。
