Taq DNA聚合酶中DNA序列的鉴定与去除。
Identification and elimination of DNA sequences in Taq DNA polymerase.
作者信息
Hughes M S, Beck L A, Skuce R A
机构信息
Department of Agriculture for Northern Ireland, Stromont, Belfast.
出版信息
J Clin Microbiol. 1994 Aug;32(8):2007-8. doi: 10.1128/jcm.32.8.2007-2008.1994.
Abstract
This study confirms that different preparations of Taq DNA polymerase are contaminated with eubacterial DNA. The contaminants appeared to represent more than one strain or species but were not identified as Thermus aquaticus or Escherichia coli. Differences in microcentrifuge tube composition appeared to affect elimination of the contaminants.
摘要
本研究证实，不同制剂的Taq DNA聚合酶被真细菌DNA污染。这些污染物似乎代表不止一种菌株或物种，但未被鉴定为嗜热栖热菌或大肠杆菌。微量离心管成分的差异似乎会影响污染物的去除。
相似文献
1
Identification and elimination of DNA sequences in Taq DNA polymerase.Taq DNA聚合酶中DNA序列的鉴定与去除。
J Clin Microbiol. 1994 Aug;32(8):2007-8. doi: 10.1128/jcm.32.8.2007-2008.1994.
2
Taq polymerase contains bacterial DNA of unknown origin.Taq聚合酶含有来源不明的细菌DNA。
Mol Cell Probes. 1990 Dec;4(6):445-50. doi: 10.1016/0890-8508(90)90003-i.
3
Removal of DNA contamination in polymerase chain reaction reagents by ultraviolet irradiation.通过紫外线照射去除聚合酶链反应试剂中的DNA污染。
Methods Enzymol. 1993;218:381-8. doi: 10.1016/0076-6879(93)18030-g.
4
Detection of DNA contamination in Taq polymerase.Taq聚合酶中DNA污染的检测。
Biotechniques. 1991 Aug;11(2):176-7.
5
Reliability of nested polymerase chain reaction in the diagnosis of bacterial endophthalmitis.巢式聚合酶链反应在细菌性眼内炎诊断中的可靠性
Am J Ophthalmol. 2002 Jan;133(1):142-4. doi: 10.1016/s0002-9394(01)01212-0.
6
7
Isolation, characterization, and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus.嗜热水生栖热菌DNA聚合酶基因的分离、特性鉴定及其在大肠杆菌中的表达
J Biol Chem. 1989 Apr 15;264(11):6427-37.
8
Elimination of contaminating DNA within polymerase chain reaction reagents: implications for a general approach to detection of uncultured pathogens.聚合酶链反应试剂中污染性DNA的去除：对检测未培养病原体通用方法的影响。
J Clin Microbiol. 1993 Mar;31(3):646-52. doi: 10.1128/jcm.31.3.646-652.1993.
9
Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli.在大肠杆菌中表达的嗜热水生栖热菌DNA聚合酶的纯化。
Anal Biochem. 1990 Dec;191(2):396-400. doi: 10.1016/0003-2697(90)90238-5.
10
Characterization of contaminating DNA in Taq polymerase which occurs during amplification with a primer set for Legionella 5S ribosomal RNA.用嗜肺军团菌5S核糖体RNA引物组扩增过程中Taq聚合酶中污染DNA的特性分析
Mol Cell Probes. 1994 Feb;8(1):11-4. doi: 10.1006/mcpr.1994.1002.
引用本文的文献
1
Cost-effective in-house COVID-19 reverse transcription-polymerase chain reaction testing with yeast-derived polymerase.使用酵母衍生聚合酶进行具有成本效益的内部新冠病毒逆转录-聚合酶链反应检测。
Ann Thorac Med. 2024 Apr-Jun;19(2):165-171. doi: 10.4103/atm.atm_180_23. Epub 2024 Apr 25.
2
Occurrence of recreational water quality monitoring general fecal indicator bacteria and fecal source identification genetic markers in gray seal scat.灰海豹粪便中休闲水质监测总粪便指示细菌和粪便来源鉴定遗传标记的出现。
Sci Total Environ. 2024 Jul 15;934:173220. doi: 10.1016/j.scitotenv.2024.173220. Epub 2024 May 18.
3
Novel rapid method for identifying and quantifying pathogenic bacteria within four hours of blood collection.新型快速方法，可在血液采集后四小时内鉴定和定量血液中的致病菌。
Sci Rep. 2024 Jan 12;14(1):1199. doi: 10.1038/s41598-023-50864-0.
4
Quantitative multiplex real-time polymerase chain reaction assay for the detection of Helicobacter pylori and clarithromycin resistance.定量多重实时聚合酶链反应检测幽门螺杆菌和克拉霉素耐药性。
BMC Microbiol. 2023 May 27;23(1):155. doi: 10.1186/s12866-023-02868-z.
5
Interlaboratory performance and quantitative PCR data acceptance metrics for NIST SRM® 2917.NIST SRM® 2917 的实验室间性能和定量 PCR 数据验收指标。
Water Res. 2022 Oct 15;225:119162. doi: 10.1016/j.watres.2022.119162. Epub 2022 Sep 24.
6
Contamination Issue in Viral Metagenomics: Problems, Solutions, and Clinical Perspectives.病毒宏基因组学中的污染问题：问题、解决方案及临床视角
Front Microbiol. 2021 Oct 20;12:745076. doi: 10.3389/fmicb.2021.745076. eCollection 2021.
7
A broad-range PCR technique for the diagnosis of infective endocarditis.一种用于诊断感染性心内膜炎的广谱聚合酶链反应技术。
Braz J Microbiol. 2018 Jul-Sep;49(3):534-543. doi: 10.1016/j.bjm.2017.03.019. Epub 2018 Feb 9.
8
Rapid Enrichment of Dehalococcoides-Like Bacteria by Partial Hydrophobic Separation.通过部分疏水分离快速富集类脱卤球菌属细菌
Appl Environ Microbiol. 2017 Mar 2;83(6). doi: 10.1128/AEM.02946-16. Print 2017 Mar 15.
9
Multicenter Evaluation of BioFire FilmArray Meningitis/Encephalitis Panel for Detection of Bacteria, Viruses, and Yeast in Cerebrospinal Fluid Specimens.BioFire FilmArray脑膜炎/脑炎检测板用于脑脊液标本中细菌、病毒和酵母菌检测的多中心评估
J Clin Microbiol. 2016 Sep;54(9):2251-61. doi: 10.1128/JCM.00730-16. Epub 2016 Jun 22.
10
Eukaryote-Made Thermostable DNA Polymerase Enables Rapid PCR-Based Detection of Mycoplasma, Ureaplasma and Other Bacteria in the Amniotic Fluid of Preterm Labor Cases.真核生物来源的热稳定DNA聚合酶可实现基于PCR的快速检测，用于检测早产病例羊水中的支原体、脲原体及其他细菌。
PLoS One. 2015 Jun 4;10(6):e0129032. doi: 10.1371/journal.pone.0129032. eCollection 2015.
本文引用的文献
1
Elimination of contaminating DNA within polymerase chain reaction reagents: implications for a general approach to detection of uncultured pathogens.聚合酶链反应试剂中污染性DNA的去除：对检测未培养病原体通用方法的影响。
J Clin Microbiol. 1993 Mar;31(3):646-52. doi: 10.1128/jcm.31.3.646-652.1993.
2
Identification of mycobacteria from animals by restriction enzyme analysis and direct DNA cycle sequencing of polymerase chain reaction-amplified 16S rRNA gene sequences.通过限制性内切酶分析以及聚合酶链反应扩增的16S rRNA基因序列的直接DNA循环测序从动物中鉴定分枝杆菌。
J Clin Microbiol. 1993 Dec;31(12):3216-22. doi: 10.1128/jcm.31.12.3216-3222.1993.
3
Frequent contamination of Taq polymerase with DNA.
Clin Chem. 1990 Jun;36(6):1258-9.
4
Taq polymerase contains bacterial DNA of unknown origin.Taq聚合酶含有来源不明的细菌DNA。
Mol Cell Probes. 1990 Dec;4(6):445-50. doi: 10.1016/0890-8508(90)90003-i.
5
Detection of DNA contamination in Taq polymerase.Taq聚合酶中DNA污染的检测。
Biotechniques. 1991 Aug;11(2):176-7.
Zotero 插件