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1
Identification and elimination of DNA sequences in Taq DNA polymerase.Taq DNA聚合酶中DNA序列的鉴定与去除。
J Clin Microbiol. 1994 Aug;32(8):2007-8. doi: 10.1128/jcm.32.8.2007-2008.1994.
2
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3
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Eukaryote-Made Thermostable DNA Polymerase Enables Rapid PCR-Based Detection of Mycoplasma, Ureaplasma and Other Bacteria in the Amniotic Fluid of Preterm Labor Cases.真核生物来源的热稳定DNA聚合酶可实现基于PCR的快速检测，用于检测早产病例羊水中的支原体、脲原体及其他细菌。
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本文引用的文献

1
Elimination of contaminating DNA within polymerase chain reaction reagents: implications for a general approach to detection of uncultured pathogens.聚合酶链反应试剂中污染性DNA的去除：对检测未培养病原体通用方法的影响。
J Clin Microbiol. 1993 Mar;31(3):646-52. doi: 10.1128/jcm.31.3.646-652.1993.
2
Identification of mycobacteria from animals by restriction enzyme analysis and direct DNA cycle sequencing of polymerase chain reaction-amplified 16S rRNA gene sequences.通过限制性内切酶分析以及聚合酶链反应扩增的16S rRNA基因序列的直接DNA循环测序从动物中鉴定分枝杆菌。
J Clin Microbiol. 1993 Dec;31(12):3216-22. doi: 10.1128/jcm.31.12.3216-3222.1993.
3
Frequent contamination of Taq polymerase with DNA.
Clin Chem. 1990 Jun;36(6):1258-9.
4
Taq polymerase contains bacterial DNA of unknown origin.Taq聚合酶含有来源不明的细菌DNA。
Mol Cell Probes. 1990 Dec;4(6):445-50. doi: 10.1016/0890-8508(90)90003-i.
5
Detection of DNA contamination in Taq polymerase.Taq聚合酶中DNA污染的检测。
Biotechniques. 1991 Aug;11(2):176-7.

Taq DNA聚合酶中DNA序列的鉴定与去除。

Identification and elimination of DNA sequences in Taq DNA polymerase.

作者信息

Hughes M S, Beck L A, Skuce R A

机构信息

Department of Agriculture for Northern Ireland, Stromont, Belfast.

出版信息

J Clin Microbiol. 1994 Aug;32(8):2007-8. doi: 10.1128/jcm.32.8.2007-2008.1994.

DOI:10.1128/jcm.32.8.2007-2008.1994
PMID:7989558
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC263920/
Abstract

This study confirms that different preparations of Taq DNA polymerase are contaminated with eubacterial DNA. The contaminants appeared to represent more than one strain or species but were not identified as Thermus aquaticus or Escherichia coli. Differences in microcentrifuge tube composition appeared to affect elimination of the contaminants.

摘要

本研究证实，不同制剂的Taq DNA聚合酶被真细菌DNA污染。这些污染物似乎代表不止一种菌株或物种，但未被鉴定为嗜热栖热菌或大肠杆菌。微量离心管成分的差异似乎会影响污染物的去除。