Miller J M, Miller M D, Driscoll P E, Miller P
Diagnostic Microbiology Section, Centers for Disease Control and Prevention, Atlanta, GA 30333.
J Clin Microbiol. 1994 Aug;32(8):2028-30. doi: 10.1128/jcm.32.8.2028-2030.1994.
Three extracting reagents were compared for effectiveness in the Ehrlich indole procedure: xylene (the recommended reagent), Hemo-De (a terpene-based product containing d-limonene), and Micro-Clear (an isoparaffinic hydrocarbon). Thirty-three strains representing 12 species of indole-positive aerobes or facultative anaerobes and 50 strains representing 11 species of indole-positive strict anaerobes were tested using the three reagents. Xylene extraction allowed indole detection in all of the isolates tested. Micro-Clear allowed detection in all of the aerobic isolates and in 49 of 50 anaerobes. Hemo-De allowed indole detection in 32 of 33 aerobes and in 49 of 50 anaerobes. There was no significant difference in the results among the reagents. Because Micro-Clear is biodegradable, nonflammable, noncarcinogenic, and odorless, we feel that this product should be considered a safe and effective substitute for xylene in the Ehrlich indole procedure.
在埃利希吲哚法中，比较了三种提取试剂的有效性：二甲苯（推荐试剂）、Hemo-De（一种含d-柠檬烯的萜烯基产品）和Micro-Clear（一种异链烷烃）。使用这三种试剂对代表12种吲哚阳性需氧菌或兼性厌氧菌的33株菌株以及代表11种吲哚阳性严格厌氧菌的50株菌株进行了测试。二甲苯提取可在所有测试分离株中检测到吲哚。Micro-Clear可在所有需氧分离株以及50株厌氧菌中的49株中检测到吲哚。Hemo-De可在33株需氧菌中的32株以及50株厌氧菌中的49株中检测到吲哚。各试剂的结果之间无显著差异。由于Micro-Clear可生物降解、不可燃、无致癌性且无气味，我们认为该产品应被视为埃利希吲哚法中二甲苯的安全有效替代品。
