Martín-Biosca Y, García-Alvarez-Coque M C, Ramis-Ramos G
Department of Analytical Chemistry, Faculty of Chemistry, University of Valencia, Spain.
J Biochem Biophys Methods. 1994 Jul;29(1):1-21. doi: 10.1016/0165-022x(94)90054-x.
The photothermal spectroscopic techniques, with special emphasis on the thermal lens spectrometry (TLS), are introduced to the non-specialist in laser spectroscopy. The following topics are treated on an elementary basis: fundamentals and analytical characteristics, instrumentation, selectivity and multi-wavelength capability, the models describing the signal-concentration relationship, the sensitivity, background noise and limits of detection, the influence of light scattering and flow. Applications related to the fields of clinical and biochemical analysis and organic pollution are given. The thermal lens circular dichroism and the infrared TLS are also briefly outlined.
本文向激光光谱学领域的非专业人士介绍了光热光谱技术，尤其着重介绍了热透镜光谱法（TLS）。以下主题将以基础方式进行讲解：基本原理与分析特性、仪器设备、选择性与多波长能力、描述信号-浓度关系的模型、灵敏度、背景噪声与检测限、光散射和流动的影响。文中还给出了与临床和生化分析以及有机污染领域相关的应用。此外，还简要概述了热透镜圆二色性和红外TLS。
