Croes K, McCarthy P T, Flanagan R J
National Poisons Unit, NHS Trust, London, U.K.
J Anal Toxicol. 1994 Sep;18(5):255-60. doi: 10.1093/jat/18.5.255.
A simple high-performance liquid chromatographic assay for the measurement of quinine, hydroxychloroquine, chloroquine, and desethylchloroquine in small volumes (50-200 microL) of serum, whole blood, or filter paper-adsorbed dry blood was developed. The analytes are isolated by liquid-liquid extraction and chromatographed on a sulfophenylpropyl-modified silica column under isocratic conditions. The column effluent is monitored by fluorimetry (excitation wavelength, 215 nm; no emission filter). The limit of quantitation is 0.005-0.01 mg/L for chloroquine and hydroxychloroquine and 0.05 mg/L for quinine. No interference from other antimalarial drugs or other compounds commonly used has been observed. Quantitative results can be obtained within 1 hour of receipt of the sample.
建立了一种简单的高效液相色谱法，用于测定小体积（50 - 200微升）血清、全血或滤纸吸附干血中的奎宁、羟氯喹、氯喹和去乙基氯喹。通过液 - 液萃取分离分析物，并在等度条件下于磺基苯丙基改性硅胶柱上进行色谱分析。通过荧光法监测柱流出物（激发波长215纳米；无发射滤光片）。氯喹和羟氯喹的定量限为0.005 - 0.01毫克/升，奎宁的定量限为0.05毫克/升。未观察到来自其他抗疟药物或常用其他化合物的干扰。在收到样品后1小时内可获得定量结果。
