Langermann S, Palaszynski S, Sadziene A, Stover C K, Koenig S
MedImmune Inc., Gaithersburg, Maryland 20878.
Nature. 1994 Dec 8;372(6506):552-5. doi: 10.1038/372552a0.
The bacillus Calmette-Guerin (BCG) is a live attenuated strain of Mycobacterium bovis which offers potential advantages as a vector for mucosal delivery of antigens. Recombinant BCG elicits protective humoral immune responses to a variety of antigens. Furthermore, BCG binds specifically to microfold cells present in the epithelium overlying lymphoid follicles throughout the mucosal immune system. Here we show that a single intranasal vaccination with recombinant BCG expressing the outer-surface protein A antigen from B. burgdorferi results in a prolonged (more than one year) protective systemic IgG response and a highly sustained secretory IgA response which is disseminated throughout the mucosal immune system. Furthermore, intranasal immunization induces marked, organized lymphocyte accumulation in the proximal nasopharyngeal lymphoid tissue as well as at distal mucosal sites; the appearance and persistence of lymphoid aggregates correlates with the secretory immune responses. Thus intranasal immunization with recombinant BCG is a powerful method for inducing long-lasting secretory and systemic immune responses.
卡介苗（BCG）是牛分枝杆菌的减毒活菌株，作为抗原黏膜递送载体具有潜在优势。重组卡介苗能引发针对多种抗原的保护性体液免疫反应。此外，卡介苗能特异性结合整个黏膜免疫系统中覆盖淋巴滤泡的上皮内存在的微褶细胞。在此我们表明，单次鼻内接种表达来自伯氏疏螺旋体的外膜蛋白A抗原的重组卡介苗，可导致延长（超过一年）的全身性保护性IgG反应以及高度持续的分泌型IgA反应，且该反应遍布整个黏膜免疫系统。此外，鼻内免疫诱导近端鼻咽淋巴组织以及远端黏膜部位出现明显的、有组织的淋巴细胞聚集；淋巴聚集物的出现和持续存在与分泌性免疫反应相关。因此，用重组卡介苗进行鼻内免疫是诱导持久分泌性和全身性免疫反应的有效方法。
