Choo Y, Sánchez-García I, Klug A
Medical Research Council, Laboratory of Molecular Biology, Cambridge, UK.
Nature. 1994 Dec 15;372(6507):642-5. doi: 10.1038/372642a0.
A DNA-binding peptide comprising three zinc-fingers has been engineered to bind specifically to a unique nine-base-pair region of a BCR-ABL fusion oncogene in preference to the parent genomic sequences. Binding to the target oncogene in chromosomal DNA is possible in transformed cells in culture, and results in blockage of transcription. Consequently, murine cells rendered independent of growth factors by the action of the oncogene revert to factor dependence upon transient transfection with a vector expressing the peptide.
一种包含三个锌指的DNA结合肽已被设计成优先特异性结合BCR-ABL融合癌基因独特的九个碱基对区域，而不是亲本基因组序列。在培养的转化细胞中，该肽能够与染色体DNA中的靶癌基因结合，并导致转录受阻。因此，因癌基因作用而变得不依赖生长因子的鼠细胞，在用表达该肽的载体进行瞬时转染后恢复为因子依赖性。
