Smith E B
Department of Clinical Biochemistry, University of Aberdeen, Foresterhill, Scotland.
Thromb Res. 1994 Aug 1;75(3):329-35. doi: 10.1016/0049-3848(94)90246-1.
Soluble fibrin/fibrinogen-related antigens and insoluble fibrin are present in virtually all samples of human aortic intima. Components of the soluble fraction were identified by SDS-PAGE and immunoblotting with specific antisera. The fibrinogen was characterized by increased proportions of low molecular mass (Mr) species (300 and 280 kD), the FDP by fragments DY and DD derived from crosslinked fibrin, and by fragment E that lacked fibrinopeptide A (FPA). Experiments suggest that fibrin is formed in situ, and free thrombin was present in all 10 samples analysed for prothrombin-related antigen (PtRA). Fibrin-derived fragment E is mitogenic, so fibrin degradation may provide continuing stimulation of smooth muscle cell (SMC) proliferation.
可溶性纤维蛋白/纤维蛋白原相关抗原和不溶性纤维蛋白几乎存在于所有人主动脉内膜样本中。通过SDS-PAGE和用特异性抗血清进行免疫印迹鉴定了可溶性部分的成分。纤维蛋白原的特征是低分子量（Mr）种类（300和280 kD）的比例增加，FDP的特征是来自交联纤维蛋白的片段DY和DD，以及缺乏纤维蛋白肽A（FPA）的片段E。实验表明纤维蛋白是原位形成的，并且在所有10个分析凝血酶原相关抗原（PtRA）的样本中都存在游离凝血酶。纤维蛋白衍生的片段E具有促有丝分裂作用，因此纤维蛋白降解可能会持续刺激平滑肌细胞（SMC）增殖。
