用Mu噬菌体DNA转染大肠杆菌。
Transfection of Escherichia coli by Mu DNA.
作者信息
Kahmann R, Kamp D, Zipser D
出版信息
Mol Gen Genet. 1976 Dec 22;149(3):323-8. doi: 10.1007/BF00268534.
PMID:799251
Abstract
Infectivity of Mu DNA was demonstrated in Ca+ +-treated Escherichia coli cells that lacked the nucleases Exo V and Endo I. The efficiency of transfection is about 10(-7) per phage equivalent. Infectivity is destroyed by denaturation of Mu DNA, and cannot be restored by renaturation.
摘要
在缺乏核酸外切酶V和内切酶I的经钙离子处理的大肠杆菌细胞中证实了Mu DNA的感染性。转染效率约为每噬菌体当量10（-7）。Mu DNA变性会破坏感染性，复性后无法恢复。
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