大肠杆菌中的基因重组：核酸外切酶I的作用。
Genetic recombination in Escherichia coli: the role of exonuclease I.
作者信息
Kushner S R, Nagaishi H, Templin A, Clark A J
出版信息
Proc Natl Acad Sci U S A. 1971 Apr;68(4):824-7. doi: 10.1073/pnas.68.4.824.
Abstract
The indirect suppression of recB(-) and recB(-)recC(-) mutations by the sbcB(-) allele is caused by the loss of a nuclease active on denatured DNA. Results from enzyme purifications and studies with a specific antiserum demonstrate that the activity present in sbcB(+) strains, and lost in sbcB(-) strains, is exonuclease I. It is likely that sbcB is the structural gene for exonuclease I. The loss of exonuclease I activity restores the recombination proficiency of Escherichia coli cells that has been lost by mutations in the recB and/or recC genes. This indicates that in the absence of the recB-recC-determined enzyme, exonuclease I prevents recombination. Hypothetical pathways illustrating this conclusion are presented.
摘要
sbcB(-) 等位基因对 recB(-) 和 recB(-)recC(-) 突变的间接抑制是由于对变性 DNA 有活性的核酸酶的缺失所致。酶纯化结果以及使用特异性抗血清的研究表明，sbcB(+) 菌株中存在而 sbcB(-) 菌株中缺失的活性是核酸外切酶 I。sbcB 很可能是核酸外切酶 I 的结构基因。核酸外切酶 I 活性的丧失恢复了因 recB 和/或 recC 基因突变而丧失的大肠杆菌细胞的重组能力。这表明在没有 recB-recC 决定的酶的情况下，核酸外切酶 I 会阻止重组。文中给出了说明这一结论的假设途径。
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