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噬菌体 Mu 突变体中的相邻插入序列 IS2 和 IS5 以及 λdarg 噬菌体中的一个 IS5。

Adjacent insertion sequences IS2 and IS5 in bacteriophage Mu mutants and an IS5 in a lambda darg bacteriophage.

作者信息

Chow L T, Broker T R

出版信息

J Bacteriol. 1978 Mar;133(3):1427-36. doi: 10.1128/jb.133.3.1427-1436.1978.

DOI:10.1128/jb.133.3.1427-1436.1978
PMID:641012
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC222181/
Abstract

Using electron microscopic heteroduplex analysis, we have demonstrated that an insertion found in a Mu prophage and in some infectious. Mu deletion-substitution mutants derived from it consists of bacterial insertion sequence IS2 linked directly to IS5. Other infectious Mu mutants derived from the same lysogen have only IS5 or a portion of IS2. In addition, we have found that an independent insertion in a transducing phage, lambda 13 dargB2, is IS5. The ends of IS5 are short, inverted duplications of each other. These observations support the notion that the DNA insertion previously designated IS5 on the basis of a single example in lambda KH100 is a bona fide bacterial insertion sequence.

摘要

利用电子显微镜异源双链分析，我们已经证明在一个Mu原噬菌体以及一些由其衍生的感染性Mu缺失 - 取代突变体中发现的一个插入片段，是由直接与IS5相连的细菌插入序列IS2组成。来自同一溶原菌的其他感染性Mu突变体仅含有IS5或IS2的一部分。此外，我们发现转导噬菌体λ13 dargB2中的一个独立插入片段是IS5。IS5的末端是彼此短的反向重复序列。这些观察结果支持这样一种观点，即先前基于λKH100中的单个例子而命名为IS5的DNA插入片段是一个真正的细菌插入序列。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba28/222181/a1f51624d752/jbacter00298-0396-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba28/222181/7d237795520f/jbacter00298-0392-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba28/222181/dcaf89e8e16b/jbacter00298-0393-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba28/222181/9e7254b78b78/jbacter00298-0394-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba28/222181/da0af2677c33/jbacter00298-0395-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba28/222181/a1f51624d752/jbacter00298-0396-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba28/222181/7d237795520f/jbacter00298-0392-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba28/222181/dcaf89e8e16b/jbacter00298-0393-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba28/222181/9e7254b78b78/jbacter00298-0394-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba28/222181/da0af2677c33/jbacter00298-0395-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba28/222181/a1f51624d752/jbacter00298-0396-a.jpg

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本文引用的文献

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Mapping of deletions and substitutions in heteroduplex DNA molecules of bacteriophage lambda by electron microscopy.通过电子显微镜对噬菌体λ异源双链DNA分子中的缺失和替换进行定位
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Heteroduplex analysis of regulatory mutations and of insertions (IS1, IS2, IS5) in the bipolar argECBH operon of Escherichia coli.大肠杆菌双极argECBH操纵子中调控突变及插入序列（IS1、IS2、IS5）的异源双链分析
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IS2, a genetic element for turn-off and turn-on of gene activity in E. coli.IS2，一种用于大肠杆菌基因活性开启和关闭的遗传元件。
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Heteroduplex structures of bacteriophage Mu DNA.噬菌体Mu DNA的异源双链结构
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Polar mutations in lac, gal and phage lambda consist of a few IS-DNA sequences inserted with either orientation.乳糖操纵子（lac）、半乳糖操纵子（gal）和噬菌体λ中的极性突变由几个以任意方向插入的插入序列（IS）-DNA序列组成。
Mol Gen Genet. 1972;119(3):223-31. doi: 10.1007/BF00333860.
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Two kinds of insertions in bacterial genes.细菌基因中的两种插入情况。
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Deletions and insertions in the immunity region of coliphage lambda: revised measurement of the promoter-startpoint distance.λ噬菌体免疫区域中的缺失和插入：启动子-起始点距离的修订测量
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