根据巨噬细胞特异性抗原F4/80和巨唾液酸蛋白的免疫组织化学定位确定分娩后1天至3个月小鼠子宫中巨噬细胞的分布。

Distribution of macrophages in the mouse uterus from one day to three months after parturition, as defined by the immunohistochemical localization of the macrophage-restricted antigens F4/80 and macrosialin.

作者信息

Brandon J M

机构信息

Department of Human Anatomy, University of Oxford, UK.

出版信息

Anat Rec. 1994 Oct;240(2):233-42. doi: 10.1002/ar.1092400210.

DOI:10.1002/ar.1092400210

PMID:7992889

Abstract

BACKGROUND

The morphology of placental detachment sites and the distribution of macrophages were studied in the mouse uterus from day 1 to 3 months post partum.

METHODS

Uterine morphology was studied using H&E and Mallory's Trichrome staining. Macrophages were identified using the macrophage-restricted antigens F4/80 and FA/11 and the leukocyte beta 2-integrin CR3 (Mac-1) which is also expressed by neutrophils.

RESULTS

The post-partum wound was infiltrated by large numbers of CR3+ F4/80+ FA/11+ macrophages, and CR3+ neutrophils. The wound resolved rapidly and no inflammatory infiltrate or necrotic tissue was seen by day 5 post partum. The area of the detachment wound and the site of the metrial gland of pregnancy resolved into a structure, here called a post-partum nodule, which persisted for at least three months. The post-partum nodule consisted of an outer layer of collagen around a group of nodule-specific cells which could be divided into haemosiderin- and lipid-bearing phenotypes. The lipid-bearing nodule cells were not found after day 20 post partum. Nodule cells were FA/11+ at all times and also expressed leukocyte common antigen. Expression of CR3 and F4/80 was found on nodule cells up to day 9-10 post partum. CR3 expression was found only occasionally after day 10. F4/80 expression was downregulated from day 9-10 to day 20 but was found consistently after day 20. Implantations of second and subsequent pregnancies are known not to occur in the immediate vicinity of post-partum nodules and nodules from successive pregnancies were seen to be separate within the uterus.

CONCLUSIONS

Since the endometrial area in the immediate vicinity of nodules is inimical to implantation and nodules consist of a group of macrophages, it can be implied that nodule macrophages are exerting some influence on the endometrium in their vicinity.

摘要

背景

研究了产后1天至3个月小鼠子宫中胎盘剥离部位的形态以及巨噬细胞的分布。

方法

采用苏木精-伊红（H&E）染色和马洛里三色染色研究子宫形态。使用巨噬细胞限制性抗原F4/80和FA/11以及也由中性粒细胞表达的白细胞β2整合素CR3（Mac-1）来鉴定巨噬细胞。

结果

产后伤口有大量CR3⁺F4/80⁺FA/11⁺巨噬细胞和CR3⁺中性粒细胞浸润。伤口迅速愈合，产后第5天时未见炎性浸润或坏死组织。剥离伤口区域和妊娠子宫蜕膜腺部位形成一种结构，此处称为产后小结节，其持续至少3个月。产后小结节由围绕一组结节特异性细胞的外层胶原组成，这些细胞可分为含铁血黄素型和含脂型。产后第20天后未发现含脂结节细胞。结节细胞始终为FA/11⁺，也表达白细胞共同抗原。产后第9 - 10天前结节细胞上可发现CR3和F4/80的表达。第10天后仅偶尔发现CR3表达。F4/80表达从第9 - 10天至第20天下调，但第20天后一直存在。已知第二次及后续妊娠不会在产后小结节紧邻区域着床，且连续妊娠的小结节在子宫内是分开的。