Lo Y M, Noakes L, Bowell P J, Fleming K A, Wainscoat J S
Niffield Department of Clinical Medicine, John Radcliffe Hospital, Oxford.
Br J Haematol. 1994 Jul;87(3):658-60. doi: 10.1111/j.1365-2141.1994.tb08336.x.
A sensitive PCR-based assay was developed to amplify fetal-derived rhesus D (RhD) sequence from peripheral blood of RhD-negative pregnant women with circulating anti-D. RhD-PCR positivity was detected in 7/22 samples from women bearing RhD-positive fetuses, despite the presence of varying levels of anti-D. Evidence is presented which suggests that rising maternal anti-D levels might reduce circulating fetal cell numbers. Further development of this assay may have implications in the clinical management of RhD-sensitized pregnancies and aid the understanding of the physiology of feto-maternal cell trafficking.
开发了一种基于聚合酶链反应（PCR）的灵敏检测方法，用于从循环抗-D的RhD阴性孕妇外周血中扩增胎儿来源的恒河猴D（RhD）序列。在怀有RhD阳性胎儿的22名女性的7份样本中检测到RhD-PCR阳性，尽管存在不同水平的抗-D。有证据表明，孕妇抗-D水平的升高可能会减少循环胎儿细胞数量。该检测方法的进一步发展可能对RhD致敏妊娠的临床管理有影响，并有助于理解胎儿-母体细胞转运的生理学。
