Lo Y M, Bowell P J, Selinger M, Mackenzie I Z, Chamberlain P, Gillmer M D, Elliott P, Pratt G, Littlewood T J, Fleming K A
Nuffield Department of Pathology & Bacteriology, John Radcliffe Hospital, Oxford, United Kingdom.
Ann N Y Acad Sci. 1994 Sep 7;731:229-36. doi: 10.1111/j.1749-6632.1994.tb55774.x.
We have developed a sensitive PCR-based assay for the RhD gene and used it to detect circulating fetal cells from RhD-positive fetuses from peripheral blood of RhD-negative mothers. With further improvement in diagnostic accuracy, this assay may have implications in the management of RhD-sensitized pregnancies in women whose partners are heterozygous for the RhD gene. Further studies are required to determine the relationship between maternal anti-D levels and circulating fetal cell numbers.
我们开发了一种基于聚合酶链反应（PCR）的灵敏检测方法用于检测RhD基因，并利用该方法从RhD阴性母亲的外周血中检测来自RhD阳性胎儿的循环胎儿细胞。随着诊断准确性的进一步提高，该检测方法可能对伴侣为RhD基因杂合子的RhD致敏妊娠妇女的管理具有重要意义。需要进一步研究以确定母体抗-D水平与循环胎儿细胞数量之间的关系。
