Sandvej K, Peh S C, Andresen B S, Pallesen G
Department of Pathology, Aarhus University Hospital, Denmark.
Blood. 1994 Dec 15;84(12):4053-60.
In this study, we have sequenced the C-terminal part of the Epstein-Barr virus (EBV)-BNLF-1 gene encoding for the latent membrane protein-1 from tissues of EBV-positive Danish Hodgkin's disease (HD) and of Danish and Malaysian peripheral T-cell lymphomas (PTLs) and from tonsils of Danish infectious mononucleosis (IM). Our study showed that some of the 7 single-base mutations and the 30-bp deletion previously detected between codons of amino acid 322 and 366 in the BNLF-1 gene of the nasopharyngeal carcinoma cell line CAO were present in all Malaysian PTLs and in 60% of the Danish PTLs. In HD and the IM cases, the mutations were present in about 30%. The 30-bp deletion and the single base mutations occurred independently, and mutations were detectable in the majority of EBV type B-positive cases. These findings suggest that the 30-bp deletion and the 7 single-base mutations in the C-terminal part of the CAO-BNLF-1 gene do not characterize a new EBV type A substrain. Rather, some of the positions of single base mutations and the 30-bp deletion are hot spots that may have mutated independently through the evolution of EBV strains.
在本研究中，我们对爱泼斯坦-巴尔病毒（EBV）-BNLF-1基因的C末端部分进行了测序，该基因编码潜伏膜蛋白-1，其来源为EBV阳性的丹麦霍奇金淋巴瘤（HD）组织、丹麦和马来西亚外周T细胞淋巴瘤（PTL）组织以及丹麦传染性单核细胞增多症（IM）的扁桃体组织。我们的研究表明，先前在鼻咽癌细胞系CAO的BNLF-1基因第322至366位氨基酸密码子之间检测到的7个单碱基突变和30bp缺失，在所有马来西亚PTL以及60%的丹麦PTL中都存在。在HD和IM病例中，这些突变的存在比例约为30%。30bp缺失和单碱基突变是独立发生的，并且在大多数B型EBV阳性病例中都可检测到。这些发现表明，CAO-BNLF-1基因C末端部分的30bp缺失和7个单碱基突变并非新的A型EBV亚株的特征。相反，单碱基突变的某些位点和30bp缺失是热点区域，可能在EBV毒株的进化过程中独立发生了突变。
