Cerny L C, Cerny E R, Barnes B, Ferreira A
Department of Chemistry, Utica College of Syracuse University, NY 13502.
Artif Cells Blood Substit Immobil Biotechnol. 1994;22(3):633-9. doi: 10.3109/10731199409117893.
To evaluate the biophysical properties of two acellular resuscitative fluids, experiments were performed using mixtures of these with suspensions of red blood cells. Two acellular resuscitative fluids were synthesized using a diacid to tetramerically stabilized hemoglobin and then complexing it with either a hydroxyethyl starch or a Tetronic Polyol. The new polymers were characterized with respect to the molecular weight, second virial coefficient and intrinsic viscosity. These fluids were then combined with red cells and the following measurements were made: non-Newtonian flow properties, malonamide induced hemolytic kinetics, sedimentation rates and oxygen transport. The in vitro evaluation indicates that these compounds are effective hemodiluents, offer protection to the red cell membrane and do not cause erythrocyte aggregation. The oxygen transport was satisfactory.
为评估两种无细胞复苏液的生物物理特性，使用这些复苏液与红细胞悬液的混合物进行了实验。使用二酸与四聚体稳定的血红蛋白合成了两种无细胞复苏液，然后将其与羟乙基淀粉或四臂聚醚多元醇络合。对新聚合物的分子量、第二维里系数和特性粘度进行了表征。然后将这些液体与红细胞混合，并进行了以下测量：非牛顿流动特性、丙二酰胺诱导的溶血动力学、沉降率和氧运输。体外评估表明，这些化合物是有效的血液稀释剂，能保护红细胞膜，且不会引起红细胞聚集。氧运输情况令人满意。
