Cerny L C, Barnes B, Fisher L, Anibarro M, Ho N, Cerny E R
Cernyland of Utica, Huber Heights, OH 45424, USA.
Artif Cells Blood Substit Immobil Biotechnol. 1996 Mar;24(2):153-60. doi: 10.3109/10731199609118881.
A resuscitative compound in freeze-dried form has been synthesized between a modified starch and a tetremerically stabilized hemoglobin. In order to complex the hemoglobin, the starch has been prepared in mono-, di-, tri-, and tetra-aldehyde moieties. The hemoglobin was stabilized with low molecular weight diacids. Electrophoretic densitometric patterns indicate compound formation. The resulting polymers were characterized with respect to oxygen transport (biotonometry), Hill constant and P50. The in vivo evaluation indicates that these compounds are effective in exchange-transfusion experiments with rats to a level of about 85% replacement of whole blood. The final product is a cost-effective acellular resuscitative compound which can be stored in freeze-dried form at room temperature for extended periods of time. This artificial blood substitute can be reconstituted upon the addition of water.
一种冻干形式的复苏性化合物已在一种改性淀粉和一种四聚体稳定化血红蛋白之间合成。为了使血红蛋白形成复合物，淀粉已制备成具有单醛、二醛、三醛和四醛部分的形式。血红蛋白用低分子量二酸进行稳定化处理。电泳密度测定图谱表明形成了化合物。所得聚合物在氧运输（生物比色法）、希尔常数和P50方面进行了表征。体内评估表明，这些化合物在大鼠的换血实验中有效，能达到约85%全血替代水平。最终产品是一种具有成本效益的无细胞复苏性化合物，可在室温下以冻干形式长时间储存。这种人工血液替代品加水后即可复溶。
